In Vitro Micropropagation of Dendrobium chrysanthum Wall. ex Lindl. –A Threatened Orchid.
Journal Title: Scholars Academic Journal of Biosciences - Year 2014, Vol 2, Issue 1
Abstract
Abstract: An efficient protocol for seed germination and micropropagation of Dendrobium chrysanthum Wall ex Lindl. was established. Four nutrient media were used for seed germination and early protocorm development: Murashige and Skoog (MS), half –strength MS, Knudson ‘C’ (KC), and Vasin and Went (VW); combinations and alone of four plant growth regulators i.e. 6-benzylaminopurine (BAP), kinetin (KN), α-napthalene acetic acid (NAA), and indole-3-butyric acid (IBA) were studied. MS medium was found as most ideal for seed germination (98±0.48) and lowest in VW (71.12 ±0.42). 3 months old protocorm were sub cultured on fresh MS medium supplemented with different concentrations of BAP, KN, NAA, and IBA alone and in combination. After 30 days highest secondary protocorms (21.25±0.63) were observed in MS medium containing BAP (4.0µM). MS medium supplemented with 8µM IBA induced the maximum roots per shoot. After 16 days of transfer to green house the survival rate was 88%.. Keywords: Dendrobium chrysanthum, MS, protorm, micropropagation, BAP
Authors and Affiliations
Someswar Rao, Bikramjit Barman
Keywords
Related Articles
- EP ID EP377849
- DOI -
- Views 62
- Downloads 0
Prevalence of Dental Caries among 5-12 Year Old School Going Children in Urban and Rural Areas of Mahabubnagar District, Telangana, India
Dental caries is a multifactorial disease. The prevalence and incidence of it in a population is influenced by various risk factors such as age, sex, socioeconomic status, sugar exposures and oral hygiene habits. Hence,...
Study on Awareness and Acceptance towards Computer Assisted Learning (CAL) Among Undergraduate Medical Students
Abstract: Computer Assisted Learning (CAL) is a method of acquiring knowledge using electronic media which is gaining recognition among students. This requires access to computers and considerable awareness on informatio...
Influence of Environmental Factors on Tetraspores Shedding and Diurnal Periodicity in Padina tetrastromatica Hauck. (Phaeophyceae) along the Visakhapatnam Coast, East Coast of India
Studies on the tetraspores shedding were made for a period of one and half year from August 1993 to January 1995 in Padina tetrastromatica growing along the Visakhapatnam coast. Experiments were conducted on the effect o...
Influence of Artisanal Fishing gears on Fish Abundance and Species Diversity in Ferguson’s Gulf of Lake Turkana, Kenya
Abstract: Fish abundance and species diversity of gill net, purse seine and beach seine gears was assessed based on fish samples obtained from artisanal fishermen in Ferguson’s gulf. Longitudinal study design was used wi...
Isolated Tubercular Osteomyelitis of Calcaneum: A Rare Case Report
Abstract: Tuberculosis is a leading cause of morbidity and mortality in developing countries including India. Skeletal tuberculosis accounts for 1-3% of all cases of tuberculosis. 10% of osteoarticular tuberculosis affec...