IN VITRO PLANT REGENERATION OF THEVETIA NERIIFOLIA, JUSS FROM INTERNODE EXPLANTS VIA INDIRECT ORGANOGENESIS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 1
Abstract
Objective: Present study was carried out to develop an efficient in vitro protocol for micro propagation of plantlets via indirect organogenesis from internode explants of Thevetia neriifolia, a medicinal plant with immense biological potentialities.Methods: For callus induction, internodes from 4 weeks old seedlings were cultured in Murashige and Skoog (MS) medium supplemented with varying concentrations (0.5-2.0mg/L-1) of Auxins (2,4-D, IBA) and Cytokinins (KIN, BA).Results: Creamy white callus obtained initially was gradually transformed into the light green meristematic callus during subsequent subculturing in most of the combinations. The best organogenic response was achieved with a combination of IBA+BA (0.5+1.0mg/L-1) with 2.722±0.752 shoots per explant. Shoot initiation was induced/enhanced by transferring the non meristematic IBA+KIN calluses to the above IBA+BA medium. However, better response for maximum shoot proliferation (6.091±1.231) was achieved when cytokinin BA (1.0mg/ L-1) was supplied individually, with 100% efficacy. Good roots were produced in IBA (1.0mg/L-1) medium and the rooted plantlets were transferred to paper cups and acclimatized (72%) subsequently in green houses.Conclusion: This rapid and successful protocol for micro-propagation definitely facilitates to promote the mass cultivation of this potent medicinal plant.Â
Authors and Affiliations
Nesy Ibrahim, Jose Padikkala, Lizzy Mathew
Keywords
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