In vitro Propagation of Caralluma diffusa (Wight) N.E.Br
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 2
Abstract
An efficient protocol is described for the rapid in vitro multiplication of an endangered medicinal plant, Caralluma diffusa, via enhanced axillary bud proliferation from nodal explants collected from young shoots of six-months-old plant. The physiological effects of growth regulators Benzylamino purine (BAP), Thidiazuron (TDZ), Kinetin (KIN), α-Naphthalene acetic acid (NAA) full strength of Murashige and Skoog’s (MS) medium on in vitro propagation were investigated. The highest number of shoots in initiation (4.16±0.30) and in subculture (17.33±0.33) and the maximum average shoot length (4.17±0.03cm) were recorded on MS medium supplemented with BAP (4.44 μM) and TDZ (0.90 μM) at pH 5.8. Rooting was best achieved on MS medium augmented with NAA (5.37 μM). The plantlets regenerated in vitro with well-developed shoot and roots were successfully established in pots containing decomposed coir waste, vermiculite and garden soil (1:1:1ratio) and grown in a shade house with 93.83% survival rate.
Authors and Affiliations
K. Kalimuthu, R. Prabakaran, T. Sasikala
Keywords
Related Articles
- EP ID EP237198
- DOI 10.9734/BBJ/2014/7488
- Views 124
- Downloads 0
Evaluation of the Effects of Drying Methods on Phytochemicals in Thevetia neriifolia Parts as a Potential Rodenticide
Synthetic rodenticides are effective and rapid in controlling rats; however, they are toxic to non-target species including humans, as well as the environment. Thevetia neriifolia (Pers.) K. Schum different parts however...
The Effect of Locally Fabricated Pelletizing Machine on the Chemical and Microbial Composition of Organic Fertilizer
In order to make organic fertilizer more quantifiable and attractive to farmers, a pelletizing machine was fabricated locally to produce pellets from powdery fertilizer. However, concerns have been raised on the potentia...
L-Phenylalanine and L-Tyrosine Catabolism by Thermophilic Geobacillus stearothermophilus
Aims: This study investigated the potential of soil thermophile Geobacillus stearothermophilus for the biotransformation of phenylalanine and tyrosine. Study Design: G. Stearothermopilus grows well at 65ºC and has a goo...
Stearoyl-ACP Delta 9 DesaturaseMight not be the only Factor Affecting Unsaturated Fatty Acids Concentration in Oil Palm (Elaeis Sp.)
Oil palm (Elaeis guineensis) is currently the major oil-producing plant in the world. E. oleifera is another species of oil palm that is not widely cultivated due to lower productivity. Oil extracted from E. guineensis s...
Growth Inhibition of Some Phytopathogenic Bacteria by Cell-Free Extracts from Enterococcus sp
Aims: To inhibit of bacterial growth of three important phyto-pathogenic bacteria: Erwinia carotovora, Clavibacter michiganensis sp. michiganensis and Xanthomonas axonopodis by cell-free extracts from submerged cultures...