IN VITRO PROPAGATION OF PRUNUS LAUROCERASUS L.
Journal Title: Natural Resources and Sustainable Development - Year 2016, Vol 6, Issue 1
Abstract
The study purpose was to initiate Prunus laurocerasus Novita in vitro cultures from minicuttings and in vitro rootedness process stimulation with activated charcoal. Initiating the in vitro culture plant heavily depends by the donor plant health, respectively by its endogenous contamination with various microorganisms. Prunus laurocerasus initiation from minicuttings was successful on MB-MS + G, solidified culture medium, with 0.1 mg / l IBA and 2 mg / l BA and optimal time for sodium hypochlorite 5% plus Tween 20 disinfections was 12 minutes. Explants provided from polluted with dust (roadside - curtains of dust) area had endogenous infections and were impossible to initiated in vitro, compared with explants from an unpolluted area (protected). A fact still unclear occurred on MS medium with added charcoal 2%, namely changing the pH value from 5.7 to 8. We suppose that change favoured the growth of certain bacteria (already in the growth chamber), and the entire culture of this experimental lot loss. In the control group, with no activated charcoal, the pH was maintained at levels similar to those of the initial and bacterial infections have not been shown. We intend to continue research on pH modification during in vitro culture and identify bacterial species.
Authors and Affiliations
Crina Georgiana Cuc, Adriana Petruș-Vancea
Keywords
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