INCIDENCE OF METHICILLIN RESISTANT Staphylococcus aureus IN THE MILK OF SURTI GOATS
Journal Title: International Journal of Agriculture Sciences - Year 2017, Vol 9, Issue 2
Abstract
Staphylococcus aureus is found pathogenic to man and animals and it is responsible for food borne outbreaks, too. The goat milk is gaining popularity due to many health benefits on human health. To know carriage of Staph. aureus in the milk of Surti goats reared in Livestock Research Station, NAU, Navsari as well as from two rural goat rearing pockets in southern Gujarat, i.e., Amalsad (Dist. Navsari) and Dharampur (Dist. Valsad), the present investigation was carried out. All the 37 isolates S. aureus isolates were subjected to antibiotic susceptibility testing against 9 selected antibiotics by agar disc diffusion method. The results showed S. aureus isolates were resistant to Cephalexin (62.16%), Methicillin (40.54%), Ampicillin (18.92%) and Kanamycin (2.70 %). All Methicillin Resistant Staphylococcus aureus (MRSA) strains identified by antibiotic sensitivity test, were subjected to PCR to detect methicillin resistant gene mecA. Agarose Gel electrophoresis revealed single compact band of 533 bp, indicative of successful amplification of target mecA gene from the genomic DNA of S. aureus. Out of 37 S. aureus, 15 isolates amplified 533 bp of mecA gene, result show that 40.54 per cent S. aureus isolates carried mecA gene.
Authors and Affiliations
S. A. PATEL
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