Induction effectiveness of acrosome reaction in prepared human spermatozoa assessed using the CD46 surface antigen
Journal Title: European Journal of Medical Technologies - Year 2015, Vol 10, Issue 1
Abstract
The semen used for artificial insemination must be subject to appropriate preparation, which involves separating sperm from seminal plasma and selecting those having the greatest reproductive capacity. One way of preparation was the centrifugation of semen by means of Percoll gradient. Our study was part of a larger research program, in which we examined the properties of the different techniques of sperm preparation. The aim of this study was to investigate whether the technique used for the preparation of semen on the basis of Percoll gradients affects the quality and the occurrence of acrosome reaction in human sperm. The study included 30 ejaculates obtained from patients ranging 21-53 of age. The samples of ejaculate were divided into two equal volumes. The first group was subject to the preparation with different Percoll gradients and the second group was without preparation. The induction of acrosome reaction with the calcium ionophore was performed on both group. Preparation of sperm with a gradient of Percoll significantly improved semen parameters such as motility, density and percentage of morphologically normal spermatozoa, but negatively affected the occurrence of the human sperm acrosome reaction. Preparation of the Percoll gradient was not practical in clinical use. Viability (VIAB) was the only practical parameter in the case of usefulness of classical semen analysis in predicting the ability of sperm to achieve the state of capacitation.
Authors and Affiliations
Grzegorz Bakalczuk, Artur Wdowiak, Magdalena Lewicka, Sava Perovic, Juric Anton, Miroslaw A. Kielak
Keywords
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