Influencing factors of the repeat reactive (RR) rates of minipools implicated in minipool(MP) nucleic acid testing(NAT) in Xiamen
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 7
Abstract
Objective To analyze the influencing factors of the repeat reactive (RR) rates of minipools implicated in minipool (MP) nucleic acid testing(NAT) in Xiamen Blood Center, in order to provide reference for NAT. Methods Samples of blood donors from January 1, 2019 to October 31, 2022 were collected in Xiamen Blood Center and tested by MP-NAT(pools of six). Statistical analysis and comparison of MP-NAT RR rates was performed among different years, testers, reagent batches, instrument combinations, CT values of MP-NAT reactive pools and sample backgrounds. Results A total of 234 715 blood samples were tested by MP-NAT, and 428 pools were reactive, in which 248 pools were individual-donor NAT reactive, with a MP-NAT RR rate of 57.9%. The difference of MP-NAT RR rates were not statistically significant among different years, testers, reagent batches, instrument combinations, and sample backgrounds (P> 0.05). The difference of MP-NAT RR rates among different CT values of MP-NAT reactive pools was statistically significant (χ2=69.587, P<0.05). Significantly abnormal RR rate accurred in two months in 2022, and returned to normal after timely handling. Conclusion The MP-NAT RR rates is one of the important indicators to monitor the quality of NAT. Once there is a significant change in the MP-NAT RR rates, comprehensive analysis and timely handling should be carried out to ensure the quality of blood detection.
Authors and Affiliations
Jiahuang WU, Shucheng ZHUANG, Bin PEI, Shanhai OU
Keywords
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- EP ID EP738586
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.07.007
- Views 57
- Downloads 0
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