Inhibitory and killing activities of black tea (Camellia sinensis) extract against Salmonella enterica serovar Typhi and Vibrio cholerae O1 biotype El Tor serotype Ogawa isolates
Journal Title: Jundishapur Journal of Microbiology - Year 2011, Vol 4, Issue 2
Abstract
Introduction and objectives: Previous studies have shown the potential antibacterial activity of black tea, Camellia sinensis, extract against many pathogenic bacteria including Gram-positives (Listeria monocytogenes and Staphylococcus aureus) and Gram-negatives (Vibrio cholerae and Salmonella enterica serovar Typhi). The aim of the current study was to assess the in vitro antibacterial activity of C. sinensis ethanolic extract (CAS) against multidrug-resistant (MDR) clinical isolates of S. Typhi and V. cholerae O1 biotype El Tor serotype Ogawa (V. cholerae Ogawa ). Materials and methods: The zone diameter of inhibition (ZDI) and minimum inhibitory concentration (MIC) values of CAS for S. Typhi and V. cholerae Ogawa isolates were determined by agar diffusion and agar dilution techniques. Bacterial killing studies were carried out in order to assess the bactericidal activity of CAS against S. Typhi and V. cholerae Ogawa, from Kolkata, India. Results: For the S. Typhi and V. cholerae Ogawa isolates, the ZDIs of CAS ranged 12-17mm and 13-21mm, respectively, and the MICs of the extract were 400-600µg/ml and 200-600µg/ml, respectively. The CAS had bactericidal action at concentrations 512µg/ml and 256µg/ml, respectively for S. Typhi and V. cholerae Ogawa. Conclusions: The black tea (C. sinensis) extract could be useful in combating emerging drug-resistance among enteropathogens including S. Typhi and V. cholerae Ogawa.
Authors and Affiliations
Shyamapada Mandal, Manisha DebMandal, Nishith Kumar Pal, Krishnendu Saha
Keywords
Related Articles
- EP ID EP155191
- DOI -
- Views 134
- Downloads 0
Induction of mutation in Bacillus subtilis lipase gene using error-prone PCR
Introduction and objective: Directed evolution is an incredibly powerful tool for altering the properties of enzymes. This approach uses a “sloppy” version of PCR, in which the polymerase has a fairly high error rate to...
A study of the prevalence of intestinal parasitic infection in HIV positive individuals in Mashhad, Northeast Iran
Introduction and objective: Intestinal parasitic infection is an important problem in the Human Immunodeficiency Virus (HIV)-infected patients. The aim of this study was to investigate the prevalence of intestinal parasi...
Laboratory study of anticandidal activity of thyme, pennyroyal and lemon essential oils by micro dilution method
Introduction and objective: Patients with candidiasis could be treated with antifungal drugs. Due to side effects and drug resistance, many studies have been conducted to investigate essential oils’ antifungal effects. T...
Detection of Salmonella strain by rapid-cycle multiplex PCR
Introduction and objective: Salmonellosis is responsible for large numbers of infections in both humans and animals. Conventional methods of isolation of Salmonella strains take 4-7 days to complete and are therefore lab...
Molecular epidemiology of Echinococcus granulosus strains in domestic herbivores of Lorestan, Iran
Introduction and objective: Hydatidosis is one of the most important common zoonosis diseases in most parts of the world. This parasite causes many damages to animal husbandry. Several strains of this parasite have been...