Inhibitory effects of bilberry extract on oxidative damage and angiogenesis in RPE cells
Journal Title: Eye Science (Yanke Xuebao) - Year 2024, Vol 39, Issue 2
Abstract
[Objective:] To investigate the inhibitory effects of bilberry extract on oxidative damage and angiogenesis in retinal pigment epithelial (RPE) cells. [Methods:] Human RPE cells (ARPE-19) were divided into control, model and bilberry extract groups. The model group was treated with 0.5 mmol/L SIN-1 for 24 h, and the bilberry extract group was treated with 10ng/bilberry extract for 1 hour, followed by 0.5 mmol/L SIN-1 for 24 h. Cell viability was measured by CCK-8. Level of reactive oxygen species (ROS) was determined using flow cytometry. Human umbilical vein endothelial cells (HUVEC) were divided into three groups, control group, model group and bilberry extract group. The model group was treated with 10 ng/mL VEGF for 8 h, and the bilberry extract group was treated with 10 ng/mL bilberry extract for 12 h, followed by 10ng/mLVEGF for 8 h. Cell migration and invasion were measured by wound healing assay and Transwell assay. Cell angiogenesis was determined by tube formation assay. [Results:] Bilberry extract had no obvious toxicity to ARPE-19 cells (≤10 ng/mL). After SIN-1 treatment, the the viability of ARPE-19 cells was significantly reduced, while incubation with 10 ng/mL bilberry extract could restore cell activity to the normal levels (P<0.001). This concentration was selected for subsequent experiments. Additionally, bilberry extract reduced the level of ROS in SIN-1-induced ARPE-19 cells (P<0.000 1). VEGF treatment significantly enhanced the migration and invasion of HUVEC, while pretreatment with bilberry extract attenuated these effects (P<0.001 0). Meanwhile, bilberry extract could significantly inhibit VEGF-induced tube formation in HUVEC (P<0.000 1). [Conclusion:] Bilbery extract possess strongantioxidant and anti-angiogenetic activities, suggesting its potential as treatment agent for AMD.
Authors and Affiliations
Xiaowen LIU, Chen CAO, Yingxue SU, Yandong WANG
Keywords
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- EP ID EP764721
- DOI 10.12419/24040602
- Views 21
- Downloads 0
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