Inhibitory Effects of Plant Extracts and Putative Endophytic Actinomycetes from the Selected Members of Meliaceae Family against MDR Pseudomonas aeruginosa
Journal Title: European Journal of Medicinal Plants - Year 2015, Vol 10, Issue 4
Abstract
Aims: To isolate and screen endophytic actinomycetes and plant extracts from Azadirachta indica and Melia azedarach for antibacterial effects against MDR Pseudomonas aeruginosa. Study Design: Isolation and identification of the endophytic actinomycetes from plant tissues, cultivation of the selected strains, solvent extraction, screening of methanolic extracts for antibacterial activity against MDR Pseudomonas aeruginosa, chemical profiling of the extracts. Place and Duration: Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan, during July 2014 to June 2015. Methodology: The endophytic actinomycetes were isolated by surface sterilization of the plant tissues. The methanolic crude extracts of the endophytic actinomycetes and extracts of plant tissues were prepared. Antibiotyping of the Pseudomonas aeruginosa was done using a panel of 13 antibiotics by disc diffusion assay. The antimicrobial activity of the methanolic extracts was determined by well diffusion assay, while the cytotoxicity of the extracts was determined by brine shrimp microwell cytotoxicity assay. The chemical profiling of the extracts was performed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC/UV) analysis. Results: 20 endophytic actinomycetes strains were isolated and screened from the selected plants, among them 17 strains were found to be active against MDR Pseudomonas aeruginosa, with low cytotoxicity against Artemia salina. Antibiotics resistance profiling of the selected 21 Pseudomonas aeruginosa strains confirmed them as multidrug resistant (MDR) strains based on their multiple antibiotics resistance (MAR) values. Metabolic profiling of the extracts through, thin layer chromatography (TLC) and HPLC exhibited an impressive chemical diversity of the active constituents in the endophytic actinomycetes and plant extracts. Conclusion: The antibacterial activity of methanolic crude extracts of endophytic actinomycetes and plant tissues against MDR Pseudomonas aeruginosa depicted the selected plants (Azadirachta indica and Melia azedarach) as a promising source of commercially useful compounds to treat infections caused by MDR Pseudomonas aeruginosa.
Authors and Affiliations
Ashba Hassan, Imran Sajid
Keywords
Related Articles
- EP ID EP350213
- DOI 10.9734/EJMP/2015/22549
- Views 128
- Downloads 0
Microtuberization of Ceropegia pusilla Wight and Arn. an Endangered Medicinal Plant
An efficient micropropagation (direct & indirect) and microtuberization protocol for an endemic and ethanomedicinal plant Ceropegia pusilla is described. The highest number of shoot initiation (3.50 0.34) and higher ave...
Use and Awareness of Medicinal Plant and Phytotherapy among Undergraduate Students at School of Pharmacy - University of Chieti, Italy
Aims: To investigate the awareness and the use of herbal products among undergraduate students of the School of Pharmacy in Chieti. Study Design: From October 2013 to December 2013, students of the School of Pharmacy at...
Contraceptive and Phytochemical Profile of Lime-Yellow Pea (Macrotyloma axillare, E. Mey) Verdc: A Tropical Climber
Background: Rising human population has detrimental effects on quality of life and lead to increased cases of pollution and poverty. Modern forms of contraceptives also have severe side effects and result in high cases o...
In vitro Membranous activity of Biosynthesized Gold Nanoparticle from Aqueous Leave Extract of Nelsonia canescens
Nano drug delivery technologies have advantages of transporting the drug to target site to better enhance its effectiveness, bioavailability and reduces side effects with dose frequency. In this study, the reducing capab...
Isolation and Chemical Structural Characterization of the Mixture of Two Related Phytosterols from Ricinus communis L. (Euphorbiaceae) Leaves
Aim: Ricinus communis, a member of the family Euphorbiaceace, is widely used in South African traditional medicine for the treatment of wounds, sores and boils. The plant was selected based on a high antibacterial activi...