Investigation of Antioxidant Potential of Methanolic Extract of Swertia chirata Buch. Ham.
Journal Title: European Journal of Medicinal Plants - Year 2014, Vol 4, Issue 11
Abstract
Aim: To evaluate the in vitro antioxidant potential and total phenolic contents of the methanolic extract of Swertia chirata. Place and Duration: Department of Botany, Dr. H. S. Gour University (HSGVV), Sagar, between February 2010 to July 2010. Methodology: The plant material (aerial part) was subjected to defatting with petroleum ether then successively extracted with methanol. Total phenolic contents of methanolic extract was determined using the Folin-Ciocalteau reagent method while in vitro antioxidant potential was evaluated by using DPPH, hydroxyl radical, nitric oxide radical scavenging as well as ferric reducing power assays. Results: The total phenolic content in 1 mg of methanolic extract of S. chirata was equivalent to 4.5 µg Catechol. The IC50 value of the DPPH method, hydroxyl radical and nitric oxide radical scavenging activity was 222.74±0.19, 307.93±0.10 and 870.55±0.20 µg/ml respectively. When these IC50 value compared with that of standard drug Butylated hydroxy anisole (BHA) the result obtained was as follows: FRSA-SCM>BHA (p=0.000246); HRSA-SCM<BHA (p=0.000507); NORA-SCM<BHA (p=2.22614). These results showed that the S. chirata methanolic extract exhibited significant free radical DPPH scavenging activity and Hydroxyl radical scavenging activity while it exhibited non-significant Nitric oxide radical scavenging activity. In ferric reducing power various concentrations (100, 250 and 500 µg/ml) of methanolic extract of S. chirata showed absorbance 0.013±0.31, 0.156±0.12 and 0.298±0.14. Phytochemical screening showed the presence of phenolic compounds such as flavonoids and tannins which may be responsible for the activity. Conclusion: Methanolic extract of Swertia chirata showed significant antioxidant activity which suggest the extract may act as a natural antioxidant agent offering effective protection from free radicals.
Authors and Affiliations
Laxmi Ahirwal, Siddhartha Singh, Manish Kumar Dubey, Vandana Bharti, Archana Mehta
Keywords
Related Articles
- EP ID EP350322
- DOI 10.9734/EJMP/2014/8933
- Views 155
- Downloads 0
In vitro Membranous activity of Biosynthesized Gold Nanoparticle from Aqueous Leave Extract of Nelsonia canescens
Nano drug delivery technologies have advantages of transporting the drug to target site to better enhance its effectiveness, bioavailability and reduces side effects with dose frequency. In this study, the reducing capab...
Pharmacological Potential of Tricosanthes tricuspidata and Clematis montana for Hypoglycemic and Antioxidant Activity
The aim of present study was to evaluate the antidiabetic and antioxidant potential of Tricosanthes tricuspidata and Clematis montana leaf and root extracts. On the basis of toxicity studies a dose of 200 mg/kg body weig...
In vitro Antiplasmodial and Antioxidant Activities of Entandrophragma cylindricum (Meliaceae) Extracts
Malaria is still regarded as a major global infectious disease in the 21st Century with a high pediatric mortality toll in the developing world. In Africa, malaria is one of the diseases causing the most morbidity and mo...
Phytochemical Screening and Antibacterial Activities of the Root Bark Extracts of Hippocratea africana (Willd.) Loes. ex Engl.
The root of Hippocratea africana (Celastraceae) is used in ethnomedicine in South-Western Nigeria to treat infectious and parasitic diseases. This study aimed at identifying the compound(s) that are responsible for the a...
Comparison of the Antioxidant Activity of Aromatic Medicinal Plants of Moroccan Origin
The purpose of this work is to appreciate different aromatic and medicinal plants from Morocco belonging to four different families, by chemical characterization and evaluation of their antioxidant activity. The reducing...