Investigation of genetic variation of lentil lines using random amplified polymorphic DNA (RAPD) and intron-exon splice junctions (ISJ) analysis
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2012, Vol 3, Issue 3
Abstract
In order to investigation of genetic variation among lentil lines and genetic relationship these to Cistan local cultivar, DNA of 20 lentil lines and Cistan local cultivar were analysed using random amplified polymorphic DNA (RAPD) and intron-exon splice junctions (ISJ) markers. PIC, MI, percentage of polymorphic bands (P), Nei , s gene diversity (H), Shannon, s information index (I) were calculated for both random, semi random and combined of random and semi random primers.
Authors and Affiliations
Salehe Ganjali*| Zabol, University of Zabol, faculty of agriculture, department of plant breeding and biotechnology,salehe.ganjali@yahoo.com, Barat Ali Siahsar| Zabol, University of Zabol, faculty of agriculture, department of plant breeding and biotechnology, Maryam Allahdou| Zabol, University of Zabol, faculty of agriculture, department of plant breeding and biotechnology
Keywords
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