Investigation of the antioxidant activity of hesperidin against 6-hydroxydopamine-induced cell damage in SH-SY5Y cells
Journal Title: National Journal of Physiology, Pharmacy and Pharmacology - Year 2018, Vol 8, Issue 6
Abstract
Background: Parkinson’s disease (PD) is regarded as a neurological condition in which continuous degeneration of dopaminergic neurons occurs selectively. Currently, most treatments for neurodegenerative diseases are palliative. According to in vitro and in vivo models of PD in recent studies, hesperidin (Hsd) showed protective properties during neuron damage. Moreover, recent reports demonstrated the induction of Hsd. Aims and Objectives: The current study aimed at analyzing the protective effect of Hsd, as a major flavanone constituent by determining its effect on 6-hydroxydopamine (OHDA)-mediated oxidative stress. The current study analyzed the impact of Hsd on neurotoxicity, mediated by 6-OHDA, in SH-SY5Y cells by an in vitro model of PD. Materials and Methods: The study employed 150 μM of 6-OHDA to induce cellular damage. Furthermore, 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide test was performed to analyze cellular viability. Fluorescence spectrophotometry was performed to measure the level of intracellular reactive oxygen species (ROS) and intracellular calcium. Based on the findings, 6-OHDA could reduce cell viability. Results: Moreover, intracellular ROS, intracellular calcium, and DNA fragmentation vastly improved in cells exposed to 6-OHDA. SH-SY5Y cell incubation with Hsd (1 and 10 μg/mL)-induced protective effects and decreased the biochemical markers of cell apoptosis. According to the findings, Hsd showed protective features against neurotoxicity, caused by 6-OHDA. These protective properties were accompanied by anti-apoptotic features. Conclusion: It was revealed that Hsd affected the management of PD. Given the preserved mitochondrial function of Hsd , and its antioxidant and anti-apoptotic properties in neuroblastoma cell lines, this compound has neuroprotective effects on 6-OHDA
Authors and Affiliations
Mehrdad Jahanshahi, Leila Elyasi, Hamed Ghazvini, Emsehgol Nikmahzar, Shohreh Taziki
Keywords
Related Articles
- EP ID EP489837
- DOI 10.5455/njppp.2018.8.1249105022018
- Views 99
- Downloads 0
A study on polypharmacy among elderly medicine in-patients of a tertiary care teaching hospital of North India
Background: In the modern era, with the increase in the lifespan of individuals with increasing access to health-care facilities, the population of elderly is also increasing with an equal pace. However, on the other han...
Evaluation of diuretic, saluretic and natriuretic activity of hydrochlorothiazide in combination with misoprostol in Wistar rats
Background: Renal vasodilatation by prostaglandins is well proven facts. Furosemide is better diuretics due to their additional effect on intrarenal hemodynamic changes brought about by increase in local prostaglandin sy...
Sympathetic function tests during pre‑ and post‑menstrual phases in young women
Background: Women undergo many types of behavioral and hormonal changes, especially during reproductive life. Besides reproductive changes, there occur cyclical fluctuations in various functions of body systems. Autonomi...
tudy of gender variation in muscle function among young adults
Background: Muscles are the only tissue in the body that has the unique ability to contract which helps in movement of other body parts. Physical activity is one of the important factors which affect the skeletal muscle...
Effect of Carica papaya leaf extract on platelet count in chemotherapy-induced thrombocytopenic patients: A preliminary study
Background: Chemotherapy-induced thrombocytopenia (CIT) is a detrimental side effect of cancer chemotherapy. Several pharmacologic agents have been evaluated, but their thrombopoietic activity is modest and often associa...