INVESTIGATION OF THE LIPID AND CARBOHYDRATE CONTENTS OF GREWIA TENAX FORSSK. FRUITS & EVALUATION OF HEPATOPROTECTION ACTIVITY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 5
Abstract
Objective: Grewia tenax Forssk. is represented in Egypt and known for its edible fruits which are nutritionally balanced and rich in iron and calcium. This work was to investigate the Lipid and Carbohydrate Contents of Grewia tenax Forssk. fruits and evaluation of Hepatoprotection activity.Methods: Investigation of carbohydrate contents of Grewia tenax Forssk. fruits were carried out using HPLC. The lipids content of Grewia tenax Forssk. fruits were analyzed by GLC. Also, the hepatoprotective activity on rat hepatocytes monolayer culture was studied.Results: The HPLC analysis of free sugars of Grewia tenax Forssk. fruits revealed the presence of stachyose, sucrose, glucose and fructose. While the HPLC analysis of the polysaccharide hydrolysate from Grewia tenax Forssk. fruits showed the presence of glucuronic acid, glucose, galactose and arabinose.GLC of the unsaponifiable matter of hexane extract of Grewia tenax Forssk. fruits revealed the presence of a mixture of a series of n-alkanes (63%) and a series of sterols (9.9%). A series of hydrocarbons ranging from C10-C26 in addition to cholesterol, campasterol, stigmasterol and β-Sitosterol and six fatty acids among which Linoleic acid (C18:2) was the major one (55.5 %) was identified. Also, the hepatoprotective activity study of the extracts of Grewia tenax Forssk. fruits (80% MeOH extract of defatted powder) and water extract indicated that they exhibited hepatoprotection at (25 μg/ml and 50 μg/ml) and (12.5 μg/ml to 100 μg/ml); respectively.Conclusion: Our study was the first record of carbohydrate and lipid fraction contents of Grewia tenax Forssk. fruits. From the obtained results, the highly nutritive and medicinal values of Grewia tenax Forssk. fruits should be considered. Furthermore, and due to the high hepatoprotection effects of the plant extracts, further investigation and studies should be done to isolate the bioactive compounds. Â
Authors and Affiliations
Ibrahim Ahmed Saleh, Khaled Ahmed Shams, Wafaa Abdel-monaem Tawfik, Amira Abdel-azim Habib, Rasmia A. Hassan, Abdelaaty Abdelaziz Shahat, Elsayed Ahmed Aboutabl, Faiza Mohamed Hammouda, Nahla Sayed Abdelazim
Keywords
Related Articles
- EP ID EP578803
- DOI -
- Views 88
- Downloads 0
IN-VITRO IN-VIVO STUDIES OF LAMOTRIGINE TABLETS PREPARED BY HOT MELT EXTRUSION TECHNIQUE
Objective: Present study deals with improvement in dissolution and bioavailability of poorly water soluble Lamotrigine (Lamo), an antiepileptic drug (AED) of phenyltriazine class. Formulation of poorly water soluble drug...
PRODUCTION AND OPTIMIZATION OF CHOLESTEROL OXIDASE FROM RHODOCOCCUS SPECIES
Objective: Optimization of conditions for cholesterol oxidase production by the microorganism isolated from urban compost and dairy soil samples.Methods: Isolates were obtained on the basis of their capability of growing...
THE EFFECT OF SOLID LIPID NANOPARTICLES ON TAMOXIFEN-RESISTANT BREAST CANCER CELLS
To overcome the acquired Tamoxifen (Tam) resistance in Tam-resistant breast cancer cells without damaging normal cells, we have examined the therapeutic efficacy of Tam-loaded solid lipid nanoparticles (SLNs). Tam-loaded...
STABILITY INDICATING RP-HPLC-PDA METHOD FOR DETERMINATION OF ABIRATERONE ACETATE AND CHARACTERIZATION OF ITS BASE CATALYZED DEGRADATION PRODUCT BY LC-MS
Objective: The present work describes stability indicating (SI) RP-HPLC-PDA method for determination of abiraterone acetate (ABA) and characterization of its base catalyzed degradation product by LC-MS.Methods: The separ...
EVALUATION OF FREE RADICAL SCAVENGING ABILITY AND ANTIRADICAL ACTIVITIES OF XIMENIA CAFFRA FRUIT EXTRACTS AT DIFFERENT RIPENING STAGES
Objective: To evaluate the free radical scavenging ability and antiradical activities of Ximenia caffra fruit extracts in their different ripening stages. Methods: Using standard procedures, Ximenia caffra fruit extract...