INVITRO ANTIOXIDANT ACTIVITY AND HPTLC ANALYSIS OF WITHANIA SOMNIFERA
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 6
Abstract
Withania somnifera is a well known medicinal plant having wide range of medicinal properties. HAEWS, MEWS and AEWS were comparatively standardized for the content of Withanolide D by HPTLC. All the samples were screened for the possible in-vitro antioxidant activity for 1,1-diphenyl-2-picryl-hydrazil (DPPH.) free radical scavenging, hydrogen peroxide scavenging, flavonoid content, polyphenolic contents, and lipid peroxidation effects. The Withanolide D content of HAEWS, MEWS and AEWS was found to be 0.103, 0.067, and 0.036% (w/w) respectively. The Polyphenol and flavonoid content of HAEWS was found to be 35.51, 17.68 (w/w) respectively. All the tested samples showed marked antioxidant activity in which HAEWS showed prominent activity. HAEWS showed significant (p<0.01) (DPPH) free radical scavenging (67.38%), hydrogen peroxide scavenging (61.05%) and lipid peroxidation (63.86%) , reducing power (0.93%) at the concentration of 1000μg/ml. These various antioxidant activities were compared to standard antioxidant such as ascorbic acid.
Authors and Affiliations
Makhija Manju*| M.Pharm, Rajasthan University of Health Science, Kumbha Marg , Pratapnagar, Jaipur, Rajasthan, India, Gupta Maheshkumar K| Ph.D, Principal, Kota college of Pharmacy, Kota,Rajasthan,India.
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