Isolation and characterization of stx1 and stx2 toxin-producing Escherichia coli in neonatal lambs with diarrhea in Nineveh governorate, Iraq
Journal Title: Journal of Applied Veterinary Sciences - Year 2022, Vol 7, Issue 4
Abstract
Escherichia coli causes many health problems in lambs, the most prominent of which is diarrhea, the study aimed to detect the shiga toxins genes in E.coli isolated from the suckling lambs affected with diarrhea in Nineveh governorate. 91 fecal samples were collected from suckling lambs from different areas of Nineveh governorate during February 2022.Fecal sampling was performed directly from the rectum and placed in sterile containers, then transferred directly to the research unit of the veterinary medicine faculty at university of Mosul. Standard microbiological methods including culture in EMB, MacConkey and brilliance agars along with biochemical analyzes were performed. Molecular confirmations were done using specific primer sets targeting uidA gene for E.coli and Stx1 and Stx2Â for shiga toxin respectively. The result of the present study showed that the prevalence rate of E.coli was 58.24%(53/91). Based on the PCR assay, the uidA, Stx1 and Stx2 were found in all the E.coli isolates 100% (53/53). It was concluded that the high isolation rate of E.coli from the lambs indicates lack of housing and hygienic procedures both for lactating ewesâ health and the environment.
Authors and Affiliations
A. H. Al-Sabawi,Dh. M. Jwher,
Keywords
Related Articles
- EP ID EP709619
- DOI -
- Views 45
- Downloads 0
Genetic sequence and phylogenetic analysis of Trichomonos gallinae in racing pigeons at Mosul city, Iraq
This is the first study in Mosul to use genetic sequencing technology to diagnose and document the type, strain, and genotype of Trichomonos gallinae in racing pigeon. It was distinguished by the geographical sequence at...
Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth
This work presents cultural characterization of Peste Des Petites Ruminants (PPR) virus in VERO-culture. The VERO cells are currently considered as an acceptable cell substrate to produce a wide range of viruses. This st...
Isolation, identification and sensitivity of Mannheimia haemolytica from Ewes udder with clinical mastitis
The current study aims to isolate and identify <em>M. haemolytica</em> from ewes with clinical mastitis using conventional techniques with comparative study to their resistance to several types of antibiotics for the iso...
A Review on the Potential Effective Cytoprotective Role of Ginseng in Rat's Organ
Panax ginseng is among the herbal agents traditionally utilized for thousands of years. It is used commonly as dried roots with possible multifaceted medical characteristics, including anti-inflammatory, anti-neoplastic...
Effect of Vegetable Oils as Adjuvants on Immune Response to Polyvalent Foot and Mouth Disease Inactivated Vaccine
Foot and mouth disease (FMD) is one of the most important viral diseases in Egypt and the main way for its control is sufficient vaccination. A vaccine that could improve early and long-lasting immunity by selecting the...