Isolation and identification of Escherichia coli O157:H7 isolated from Veal Meats and Butchers’ Shops in Mosul city, Iraq
Journal Title: Journal of Applied Veterinary Sciences - Year 2022, Vol 7, Issue 4
Abstract
Escherichia coli (E. coli) O157:H7 is considered a significant food-borne microorganism that causes food poisoning infections in humans every year. E. coli O157:H7 has various virulence factors such as Shiga-toxin encoding (Stx1 and Stx2). Meat and its products are considered the best meals that consumers eat every day worldwide, but meat and its products are exposed to contamination through unhygienic processing, handling, and storage. The aim of the study was the isolation of E. coli O157:H7 and detection of the uidA, Stx1, and Stx2 genes. 504 samples of meat and butchers’ shops were gathered from diverse areas in Mosul city. Classical and molecular biology techniques were used to isolate and identify E. coli O157:H7. The results appeared to indicate the total number of E. coli isolates in this study was 138 and the spread rate of E. coli O157:H7 isolated was 9.4% (13/138). The spread rate of E. coli O157:H7 was high in workers hands 4 (20%), while we did not detect E. coli O157:H7 in Machines. Additionally, all E. coli O157:H7 have the uidA, and Stx2 genes at 100%, while 92.3% of E. coli O157:H7 possess the Stx1 gene. The study concludes E. coli O157:H7 occurrences in meats and butchers’ shops and that all equipment and tools used were capable of transmitting E. coli O157:H7 to meats. Meats and butchers’ shops are a risk to humans who consume the uncooked meats.
Authors and Affiliations
Shaker Othman, Omar Sheet, Raad Alsanjary
Rapid Detection Of Bovine Herpes Virus Type 1 (Bohv-1) In Egyptian Dromedary Camels
Bovine herpesvirus 1 (BoHV-1) is known to cause reproductive disorders in Sudanese camels. Egypt imports about 90% of its camels from Sudan, and the rest from Somalia. The BoHV1 is a viral disease of bovines that can be...
A Review on Various Antioxidants Utilized in Bovine Semen Extenders
High-quality cryopreserved bovine semen is vital for successful artificial insemination (AI) program, which is the most inexpensive and the quickest mode of genetic improvement. Cryopreservation of sperm is an effective...
Comparison of tuberculin skin test and lateral flow rapid test for detection of bovine tuberculosis in dairy cattle
Tuberculosis is the most important zoonotic bacterial disease that is hazardous to both man and animals. A huge economic loss which could be direct or indirect is associated with the disease, so rapid diagnostic tests fo...
The in vitro Potential Oncolytic Effect of Lentogenic and Velogenic Newcastle Disease Viruses on MCF-7 and Caco-2 Cell Lines Compared to Chemotherapies
Cancer is the leading cause of death worldwide, with breast and colorectal cancers being the two most common cancer forms.The present work was designed to investigate the probable oncolytic effect of lentogenic and velog...
Causes and Treatment of Feline Dystocia
The objective of the present study was to describe retrospective data, including causes and whether medical and surgical management is suitable for feline dystocia. Data were analysed and described according to original...