Isolation and molecular characterization of urinopathogenic associated Klebsiella pneumoniae from urinary tract infected patients
Journal Title: World Journal of Pharmaceutical Sciences - Year 2017, Vol 5, Issue 6
Abstract
Urinary tract infection is a significant cause of morbidity and mortality. An infection occurs when tiny organisms usually bacteria from the digestive tract, cling to the urethra. A total of 120 urine samples were collected from various hospitals around the Erode district of Tamil Nadu. Out of 120 urine samples were studied, growth of bacteria was observed in 34 urine samples and no growth were seen in 86 samples they found to be healthy. The infected patients were found to be the 15 to 37 years old. The urine samples showed gram negative rod shaped bacteria, non-motile and capsulated bacteria. The respective cultural morphology, biochemical tests and carbohydrate fermentation tests it clear that isolated belongs to be Klebsiella pneumoniae. The continuous observation showed that Klebsiella has replaced other predominant urinary pathogens. Antibiotic sensitivity test revealed that Klebsiella pneumoniae was resistant to six antibiotics by clinical and laboratory standards. Resistance organism has high molecular weight of about 5258bp for Klebsiella pneumoniae. Multiple restriction sites of plasmid are analyzed by using EcoR1, Hind III, BamH1 restriction enzymes and found were digested product.
Authors and Affiliations
Tariq AL
Keywords
Related Articles
- EP ID EP330263
- DOI -
- Views 76
- Downloads 0
Estimation and Investigation of Antimicrobial Activity of 5-methyl Acetyl Salicylic Acid against Two Pathogenic Fungi
The current research was carried out to estimate and investigate the antimicrobial activity of phenolic compound represented by 5- methyl acetyl salicylic acid. Six concentrations of this acid were applied for measuremen...
Comparative study of Bee Propolis from different Geographical location
Cancer is now one of the leading causes of death in developing countries, killing more people globally each year than any other. By 2020 there are expected to be 15 million new cases of cancer every year globally, 70% of...
Preliminary evaluation of antinociceptive and anti-inflammatory activities of petroleum ether extracts of Butea monosperma (L.) leaves in laboratory animals
The petroleum ether extract of the leaves of Butea monosperma was investigated for its antinociceptive and anti-inflammatory activity in respective animal models. In the present study leaves were extracted successively t...
First assessment the analytical quality specification in Clinical Chemistry laboratory, using Sigma scale and EQA/PT in Iran: a pilot study
Background: To assess the analytical performance of quality trough external quality assesses and internal quality program data on sigma scale. Method and material: Imprecision was determined from the cumulative Levey-je...
Formulation and Evaluation of nanoparticles loaded with Escitalopram using intelligent Biomaterial isolated from the fruit pulp of Ficus carica
The purpose of the present study was to formulate and evaluate Escitalopram loaded bio-nanoparticles for effective treatment of Depression. For the preparation of bio-nanoparticles biomaterial was isolated from fruits of...