Isolation, Identification and in-Silico Characterization of Bioactive Peptide from the Venom Sac of Conus inscriptus
Journal Title: International Journal of Experimental Research and Review - Year 2024, Vol 38, Issue 2
Abstract
Nature has been the source of new medications since ancient times, and at least half of all commercialised pharmaceuticals have been made from renewable resources to treat various illnesses, including pain, inflammation, and infection. In recent years, interest has risen in bioprospecting of new bioactive peptides, especially for medical and health-related applications. Therefore, the production of functional meals is starting to get recognition for its potential to enhance quality of life when combined with a healthy lifestyle. In view of this, the current work aimed to isolate, purify, and identify bioactive peptides from Conus inscriptus venom sac protein extracts, with an emphasis on antioxidant and anti-inflammatory properties. The findings reveal that the DPPH radical scavenging activity, albumin denaturation, and HRBC membrane stabilisation inhibition activities of Conus inscriptus venom sac crude protein extracts are 23.76±2.4, 66.23±3.1, and 57.32±2.6% respectively. Secondary screening of purified protein fractions obtained through sephadex G100 gel filtration exhibited increased anti-inflammatory efficacy from fractions 19 to 23. Electrophoresis and PMF analysis revealed that the purified anti-inflammatory peptide was homogenous with a molecular weight of 12 KDa, and it was identified as a conotoxin MI15b precursor. The 3D model of conotoxin exhibits the highest similarity with template c6nk9A with a confidence of 54.1%, and its function was predicted as a potassium ion channel inhibitor. The findings of the Insilco characterisation of the conotoxin MI15b precursor concluded that this protein might serve as an anti-inflammatory agent and may be responsible for therapeutic actions in the medical management of many inflammatory-related disorders.
Authors and Affiliations
Vidyalatha Jakkana, Shanti Prabha Yamala
Keywords
Related Articles
- EP ID EP734941
- DOI 10.52756/ijerr.2024.v38.001
- Views 68
- Downloads 0
Nutritional status and effect of physical activity on anthropmetric characteristics of Bengalee Muslim adolescents boys of North 24 Parganas, West Bengal, India
A cross-sectional study of 522 Bengalee Muslim boys aged 10-17 years of Deganga, North 24 Parganas, West Bengal, India, was undertaken to study their age differences in nutritional status and effect of physical activity...
Polycarboxylate Superplasticizers Used in Concrete: A review
Modern concrete frequently uses a variety of chemical admixtures, like setting time-retarding admixtures, viscosity-modifying admixtures (VMA), and superplasticizers (SPs). These chemical admixtures greatly impact cement...
Effectiveness of an Information Booklet on the Awareness of Antimicrobial Resistance (AMR) among Health Care Professionals in a Selected Primary Care Hospital
The study aimed to assess the level of knowledge on Antibiotics and its resistance among healthcare professionals and the effectiveness of an information booklet, which includes the Centers for Disease Control and Preven...
Study of Densification Behavior of SiAlONs Using Dysprosium Containing Additive System
SiAlON ceramics have complicated chemistry and should be considered a group of alloys with diverse characteristics. It is generated when silicon nitride (Si3N4), aluminium oxide (Al2O3) and aluminium nitride (AlN) react...
Experimental Investigation of Two-Pass Solar Dryer with V-Corrugated Absorber for Potato Slice Drying
Reducing the moisture level of food items can help prevent bacterial development and deterioration, extend shelf life, reduce packaging, and improve storage for convenient transportation. In this paper, a two-pass solar...