KINETIC ANALYSIS OF THE ENZYMATIC HYDROLYSIS OF FISH MUSCLE TISSUE PROTEIN
Journal Title: Вестник Астраханского государственного технического университета. Серия: Рыбное хозяйство - Year 2013, Vol 0, Issue 3
Abstract
The aim of the study is to evaluate the feasibility of the use of experimental methods of kinetic analysis of protein enzymatic hydrolysis of the complex multi-component enzyme-substrate system for the process orientation determining. The results of the experimental-theoretical studies of the kinetic process for the enzyme-substrate systems containing enzyme Protosubtilin G3X of 0.01 and 0.05 mg/g are given. The analysis of the experimental kinetic curves suggests that for both enzyme concentrations in the considered time interval hydrolysis has the character of the fading process of the retention of nitrogen of watersoluble thermally stable compounds in the hydrolyzate. The damping cause can be due to specific substrate exhausting or competitive displacement of macromolecular proteins – substrate by reaction products. To determine the cause of the decay the experimental curves, presented in the integrated Michaelis – Menten equation, were converted into Walker – Schmidt coordinates. From the transformed integral form, the values of enzymatic hydrolysis effective constants were calculated. For the enzyme-substrate systems with a low enzyme content the effective constants are negative that indicates the competition for the active sites of the enzyme between the macromolecular protein substrates and the products of their hydrolysis. For the enzymesubstrate systems with a high enzyme content such regularities are not revealed. The possibility of kinetic analysis method application for the preliminary assessment of the character and depth of the protein enzymatic hydrolysis is stated. The revealed regularities give grounds to assert that for obtaining the hydrolyzate of the forecasting content the process should be carried out at a lower dose of the enzyme. Nitrogenous substances of the hydrolyzate will be generally presented with heavy peptides.
Authors and Affiliations
Vinnov Sergeevich , Dolganova Vadimovna
Keywords
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