Kinetic and Thermodynamic Study of Catalase Enzyme in Iraqi Patients with Active Acromegaly
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 1
Abstract
Acromegaly is a rare, chronic, progressive disease characterized by an excess secretion of growth hormone (GH) and increased circulating insulin-like growth factor 1 (IGF-1) levels. It is caused by a pituitary adenoma in the vast majority of cases. Catalase is a tetrameric protein of 244 kDa, comprising four identical subunits of 59.7 kDa. Each subunit contains 527 amino acid residues. CAT is responsible for degradation of hydrogen peroxide (H2O2). It is protective enzymes that coexist in nearly all animal cells. The aim of this study is Estimation of oxidative stress in serum by measuring catalase enzyme in acromegalic group, Study of the optimum conditions of the catalase enzyme in sera of patients with acromegaly and comparing them with the optimum conditions of control group and determining the kinetic and thermodynamic parameters of catalase enzyme in acromegaly patients and comparing them to the control group. 30 male control and 30 male patients with active acromegaly was selected for this study. The results in this study revealed a significant decrease in mean serum levels of CAT activity in acromegaly group compared to control group. The optimum conditions for CAT enzyme are (65 mmol / L, pH=7.0, 45ºC) for patients and control, 80 sec for patients and 60 sec for control. Kinetic parameters of patients group (Ka, K+1, K-1 and n [Et]) are lower than in the control group and (Km and t0.5) of patients are higher when compare with control group. Thermodynamic parameters (ΔHo, ΔSo, ΔH*, ΔG* are positive and ΔGo, ΔS* are negative) for patients and control groups. Ea for patient is higher than Ea for control group. Acromegaly patients group have large activation energy so need large time to give the product than control group. Decrease CAT enzyme affinity to substrate and rate of reaction of CAT for acromegaly patients group. CAT is a spontaneous and endothermic process through negative value of (ΔGo) and positive value of (ΔHo).
Authors and Affiliations
Hajir K. Ismail, Salma A. Abbas, Baydaa A. Abed
Keywords
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