Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 2
Abstract
Intestinal sulfate-reducing bacteria reduce sulfate ions to hydrogen sulfide causing inflammatory bowel diseases of humans and animals. The bacteria consume lactate as electron donor which is oxidized to acetate [i]via [/i]pyruvate in process of the dissimilatory sulfate reduction. Pyruvate-ferredoxin oxidoreductase activity and the kinetic properties of the enzyme from intestinal sulfate-reducing bacteria [i]Desulfovibrio piger[/i] and [i]Desulfomicrobium[/i] sp. have never been well-characterized and have not been yet studied. In this paper we present for the first time the specific activity of pyruvate-ferredoxin oxidoreductase and the kinetic properties of the enzyme in cell-free extracts of both [i]D. piger[/i] Vib-7 and [i]Desulfomicrobium[/i] sp. Rod-9 intestinal bacterial strains. Microbiological, biochemical, biophysical and statistical methods were used in this work. The optimal temperature (+35°C) and pH 8.5 for enzyme reaction were determined. The spectral analysis of the purified pyruvate-ferredoxin oxidoreductase from the cell-free extracts was demonstrated. Analysis of the kinetic properties of the studied enzyme was carried out. Initial (instantaneous) reaction velocity (V[sub]0[/sub]), maximum amount of the product of reaction (P[sub]max[/sub]), the reaction time (half saturation period) and maximum velocity of the pyruvate-ferredoxin oxidoreductase reaction (V[sub]max[/sub]) were defined. Michaelis constants (K[sub]m[/sub]) of the enzyme reaction were calculated for both intestinal bacterial strains. The studies of the kinetic enzyme properties in the intestinal sulfate-reducing bacteria strains in detail can be prospects for clarifying the etiological role of these bacteria in the development of inflammatory bowel diseases.
Authors and Affiliations
Ivan V. Kushkevych
Keywords
Related Articles
- EP ID EP81138
- DOI -
- Views 97
- Downloads 0
Enteroviruses Associated with Aseptic Meningitis in Poland, 2011–2014
A 4-year study (2011–2014) of patients with meningitis was performed. Out of the 686 cerebrospinal fluid sles, 465 (67.8%) were positive for eneteroviruses using RT-PCR and out of 334 clinical sles, 216 (64.7%) were posi...
Escherichia coli and Salmonella spp. Early Diagnosis and Seasonal Monitoring in the Sewage Treatment Process by EMA-qPCR Method
Numerous studies have recently shown that molecular biology tools can allow for early diagnosis of pathogens and can substitute existing cost and time-taking traditional methods. One of them, the qPCR, is successful...
Contents Vol. 66, 1–4, 2017
Comparison of Free and Immobilized L-asparaginase Synthesized by Gamma-Irradiated Penicillium cyclopium
Gamma irradiation is used on [i]Penicillium cyclopium[/i] in order to obtain mutant cells of high L-asparaginase productivity. Using gamma irradiation dose of 4 KGy, [i]P. cyclopium[/i] cells yielded L-asparaginase with...
<strong>Qualitative and Quantitative Characteristics of Selected Bacterial Groups in Children with Inflammatory Bowel Diseases</strong>
The aim of the study was evaluation of qualitative and quantitative changes in bacterial ecosystem in 109 children with inflammatory bowel diseases. Stools obtained from patients were analysed for selected bacteria and c...