Klebsiella pneumoniae NDM-1 prevalence and drug-sensitivity at CSK WUM between January 1, 2012 and September 30, 2014
Journal Title: Postępy Nauk Medycznych - Year 2015, Vol 28, Issue 4
Abstract
Introduction. Klebsiella pneumoniae causes nosocomial and outside-of-hospital-acquired infections. In the past years, carbapenem-producing K. pneumoniae strains, i.e. enzymes hydrolysing carbapenems, considered to be drugs of last resort in treating severe Gram-negative infections, have been more and more often identified all around the world (including Poland).Aim. The study was to retrospectively assess carbapenem-producing K. pneumoniae prevalence and antibiotic-resistance. The results of the microbiological examination of samples collected at the CSK WUM surgery unit, between January 1, 2012 and Septem ber30, 2014, were assessed.Material and methods. Together, 17 986 patient samples and 1459 rectal swabs were tested. K. pneumoniae carbapenem production was assessed with biochemical, phenotypic, and genetic techniques. The antibiotic sensitivity of the isolated K. pneumoniae strains was assessed with the disc diffusion method and the quantitative method, using bands saturated with antibiotic within the concentration gradient.Results. K. pneumoniae was the second most prevailing Enterobacteriaceae species – 236 strains were identified from samples. Sixty-seven percent K (n = 158) of K. pneumoniae produced extended spectrum beta-lactamases (ESBL) and 6% (n = 14) produced New Delhi metallo-beta-lactamase (NDM-1). All 14 K. pneumoniae NDM-1 strains were totally sensitive only to gentamicin, 12 strains were sensitive to colistin, two strains were sensitive to imipenem, and only one strain was sensitive to meropenem.Conclusions. The study results indicated the percentage of K. pneumoniae NDM-1 isolated from patients was increasing and extended spectrum beta-lactamases were prevailing (67%). All K. pneumoniae NDM-1 strains presented resistance to most available antibiotics and different phenotypes of carbapenem resistance. K. pneumoniae NDM-1 isolated from samples were confirmed to come from individuals carrying the microorganism in their gut flora.
Authors and Affiliations
Agnieszka Milner, Danuta Bieńko, Renata Kamola, Halina Marchel, Olga Saran, Grażyna Dulny, Ewa Swoboda-Kopeć
Keywords
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