Leaf as a Potential New Source of Competent Cells for High Frequency Regeneration through Indirect Organogenesis in Tylophora Indica (Burm.f.) MERRILL (Asclepiadaceae)
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2012, Vol 1, Issue 2
Abstract
The purpose of this study was to develop a new micropropagation system for Tylophora indica, an important medicinal plant in India, using leaf explants as starting material. Leaf explants cultured on Murashige & Skoog’s (MS) medium supplemented with 2mg/L NAA and 1mg/L BAP, compact greenish white callus resulted from leaf explants. From leaf explants on MS + 2mg/L 2,4-D + 0.75 mg/L BAP and MS + 2.5mg/L 2,4,5-T + 0.5 mg/L BAP, friable callus was obtained. The compact callus also yielded friable callus when transferred to B5 medium supplemented with 0.75 mg/L 2,4-D and 0.2 mg/L NAA. These calli were non morphogenetic. Morphogenetic compact callus derived from leaf explants differentiated shoots on MS + 1.0mg/L 2ip + 4mg/L KIN. Good rooting response was observed when regenerated shoots were inoculated in ½ MS with 2.0% sucrose. Such plantlets ere successfully transferred to soil after hardening, with a high rate of survival. The plants were comparable to natural population in growth in vigour.
Authors and Affiliations
Y. Justin Koilpillai| Department of Biotechnology, Sathyabama University, Chennai, India 600119.
Keywords
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