Maturation and germination of somatic embryos of Sorghum bicolor (L.) Moench cultivar ‘CIAP 132R-05’
Journal Title: Biotecnología Vegetal - Year 2017, Vol 17, Issue 1
Abstract
In sorghum [Sorghum bicolor (L.) Moench], developed protocols for plant regeneration via somatic embryogenesis do not include maturation stage. The present work was carried out with the aim of achieving the maturation and germination of sorghum somatic embryos in cultivar ‘CIAP 132R-05’. It were studied four concentrations of sucrose (30, 50, 70 and 90 g l -1 ), two of abscisic acid (0.95 and 1.90 μM) and a control without this growth regulator. Germination initiation (days) and number of somatic embryos with complete germination were evaluated in three periods (1 - 7, 8 - 14 and 15 - 21 days of culture). In addition, the effect of 6-BAP (0, 8.9, 17.8 and 26.6 μM) on somatic embryo germination was determined. The germination start time (days) and after 21 days the number of somatic embryos with complete germination and plants with malformations were determined. The addition of 70 g l -1 sucrose in the culture medium without abscisic acid increased the germination of the somatic embryos to 37.2 plants per embryo group (0.5 g of fresh mass). The highest number of somatic embryos germinated was obtained with 17.78 μM 6-BAP in the germination culture medium. It was demonstrated the need of a maturation stage in the sorghum somatic embryogenesis to increase the germination percentage.
Authors and Affiliations
Silvio de J Martínez , Rafael Gómez-Kosky , Raúl Barbón Rodríguez , Raúl Collado , Marta Pérez , Novisel Veitia , Mayelin Rodríguez
Keywords
Related Articles
- EP ID EP682985
- DOI -
- Views 178
- Downloads 1
Protocol for the isolation of intact chloroplasts from leaves Musa spp. obtained by in vitro culture and evaluation of photosynthetic activity
Chloroplasts are organelles of plant cells where photosynthesis takes place by converting light energy into chemical energy (ATP) and generating reducting power (NADPH). Isolation of chloroplasts and evaluation of phot...
Influence of agar concentration on the in vitro multiplication of Cymbopogon citratus (D.C.) Stapf
Here are presented the results on in vitro multiplication of lemon grass (Cymbopogon citratus (D. C.) Stapf.); it is a very important medicinal plant because its analgesic, antinflamatory and hipotensor properties, amon...
Effect of parameters involved in the transformation via Agrobacterium tumefaciens in the regeneration of Phaseolus vulgaris L. cultivars ‘ICA Pijao’ and ‘BAT 93’
Phaseolus vulgaris is a crop widely used in human consumption. Its production is affected by the incidence of biotic and abiotic factors. Genetic transformation provides the possibility of reducing these affections, bu...
Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities
Endophytic microorganisms live inside healthy plants and their isolation and identification may favor biotechnological applications. We isolated endophytic bacteria from Ilex paraguariensis callus cultivated in vitro f...
Field resistance of selected potato clones to Early blight
Six potato clones, selected in vitro for their resistance to Alternaria solani Sor. culture filtrates, were evaluated for their field response to early blight infection. Field screening were performance under artificial...