METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF SAROGLITAZAR IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC
Journal Title: World Journal of Pharmaceutical Research - Year 2017, Vol 6, Issue 10
Abstract
A simple, sensitive and rapid reverse phase high performance liquid chromatographic method was developed for the estimation of Saroglitazar in tablet dosage form. A C18 Inertsil ODS column (250 × 4.6 mm, 5μm particle size) was used as a stationary phase with a mobile phase containing a mixture of phosphate buffer and acetonitrile in the ratio of 40:60, v/v. The flow rate was 1.0 mL/min. The effluent was monitored at 294 nm and eluted at 2.162 min. Calibration curve was plotted with a range from 20-100 μg/ml for Saroglitazar and the correlation was found to be 0.999. The accuracy range was found between 99.95 -101.07%. The % RSD values for both intraday and interday precision were less than 2.0. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.405μg/ml and 4.260μg/ml respectively. The assay was validated for the parameters like system suitability, precision, accuracy, and robustness parameters. The proposed method can be useful for the routine determination of Saroglitazar in pharmaceutical dosage form.
Authors and Affiliations
Nita Yadav
Keywords
Related Articles
- EP ID EP618184
- DOI -
- Views 186
- Downloads 0
A REVIEW ON RESEARCH EVIDENCES IN HOMOEOPATHY FOR URINARY TRACT INFECTIONS
Urinary tract infections (UTI) are very common morbidities encountered nowdays in clinical practice. The majority of UTIs are caused by the Escherichia coli (E. coli) bacteria. The objective of this study was to review t...
COMPARATIVE STUDY OF SINENSETIN COMPOUND IN THE LEAVES OF ORTHOSIPHON STAMINEUS BENTH. FROM DIFFERENT REGIONS OF INDONESIA
The optimal growth of plant can be influenced by various factors, including the growing place. Because it could influenced the metabolites biosynthesis. This aimed study is to comparison the metabolite level, such as sin...
CLINICAL IMPORTANCE OF VYADHI SANKARA- A REVIEW
The main aim and objective of Ayurveda is to maintain the health of Swastha Purusha and to eradicate the diseases of Atura Purusha. For this purpose, the diagnosis of diseases is very important and for this purpose Nidan...
ANTIMICROBIAL ACTIVITY STUDIES, PREPARATION AND CHARACTERIZATION OF NEW AZO COMPLEXES DRIVEN FROM N-(4-HYDROXYPHENYL) ACETAMIDE
Coupling reaction of N-(4-hydroxyphenyl)acetamide with some sulfa drugs (sulfa pyrdine, Lp and sulfamethazin, Lm) gave the new bidentate azo ligand. The prepared ligands were identified by Micro elemental Analysis, 1H NM...
A CRITICAL REVIEW OF BHAISHJYARATNAVALI WITH SPECIAL REFERENCE TO MANAHSHILA (ARSENIC DISULPHIDE) AND SOMALA (ARSENIC OXIDE)
Three arsenic compounds i.e. Manashila (Realgar-Arsenic disulphide) and Somala (Arsenic oxide), are considered as the deadly poisonous compounds in Ayurveda, but these compounds after proper processing (Shodhana) have mi...