Method of propagation, stimulation of rhizomes growth in vitro culture and adaptation in the open ground for the genus Miscanthus representatives
Journal Title: Plant varieties studying and protection - Year 2017, Vol 13, Issue 3
Abstract
Purpose. To develop a method of propagation, stimulation of rhizomes growth in vitro culture for the genus Miscanthus representatives and their adaptation in the open field without the use of greenhouse complexes for acclimatization and completion of growing. Methods. Biotechnological procedures, mathematical and statistical analyses. Results. Prescription of nutrient medium was developed for explants inoculation, sprouts propagation, rhizomes growth stimulation in vitro. Such sterile explants as seeds, buds to be removed from rhizomes, parts of stems with bud were placed on modified media with mineral portion by Murashige and Skoog (MS) that contained 0,5–1 dose of macroelements and one dose of microelements, vitamins (10 mg/l of thiaminum, 1,0 mg/l of pyridoxine, 1,0 mg/l of nicotinic acid and 1,0 mg/l of ascorbic acid) supplemented with amino acids (250 mg/l of glutamic acid, 3 mg/l of tyrosine, 3 mg/l of arginine, 2 mg/l of hydroxyproline), plant growth regulators [0,5–1,0 mg/l of GA (gibberelline acid), 0,2 mg/l of 6-BAP (6-Benzylaminopurine, 0,1 mg/l of NAA (α-naphtylacetic acid)] in different variations. After seed germination, buds emerging and sprouts formation 1–2 cm in height, for propagation purpose they were passivated on the medium of other composition that differed from previous one by the content and ratio of growth regulators, especially by a high concentration of cytokinins [6-BAP (0,4–0,5 mg/l), kinetin (0,5 mg/l), adenine (0,5 mg/k)] in different variations in presence of GA (0,2 mg/l). In order to stimulate rhizomes growth, microclones were transferred on media with other composition and ratio growth regulators (6-BAP (0,2–0,3 mg/l) + GA (0,5–1,0 mg/l) or 6-BAP (0,2–0,3 mg/l) + GA (0,5–1,0 mg/l) + NAA (0,1 mg/l), in other words, with a high content of gibberellins. After the formation of rhizomes 10–15 cm in length, miscanthus plants were planted out in the open ground. Stimulation of rhizomes initiation and elongation on appropriate nutrient media before Miscanthus giganteus, M. sacchariflorus and M. sinensis planting in vivo resulted in 100% adaptation and 100% survival of plants in the winter period without the use of greenhouse complexes. Conclusions. The method of miscanthus propagation in vitro and adaptation in the open ground was developed that included stimulation of rhizomes growth and favoured the increase of their length on media supplemented with gibberelline that guaranteed 100% preservation of microplants to be propagated from in vitro culture during adaptation in the open ground and acclimatization in winter.
Authors and Affiliations
Svitlana Gontarenko, Snizhana Lashuk
Keywords
Related Articles
- EP ID EP252248
- DOI 10.21498/2518-1017.13.3.2017.110703
- Views 130
- Downloads 0
Methodical algorithm of harmonized identification of plant varieties morphological characteristics
The article performs the requirements to the preparation and Table characteristics content of the holding Test guidelines examination of new varieties to identify the difference, uniformity and stability. It is pointed t...
Drought tolerance of woody vines of the Vitaceae Juss. family under conditions of introduction in the Right-Bank Forest-Steppe of Ukraine
<p lang="en-GB" align="JUSTIFY"><span style="font-family: OfficinaSansCTT;"><span style="font-size: small;"><strong>Purpose. </strong>Investigate the drought tolerance of woody vines of <em>Ampelopsis</em> Michx and <em>...
Експресія аквапорину PIP2;1 як ознака посухостійкості гібридів Zea mays L. за умов зниженої вологості ґрунту
<p style="text-indent: 0.4cm; margin-bottom: 0cm; line-height: 0.39cm;" lang="ru-RU" align="JUSTIFY"><span style="color: #000000;"><span><span style="font-size: small;"><strong>Мета.</strong> Дослідити експресію аквапори...
Determination of commercially valuable characteristics of plant varieties for energetic use during the state examination
The analysis of commercially valuable indices of plant varieties for energetic use was carried out and the necessity to determine energetic indices during the state scientific-and-technical examination is substantiated....
Morphological features and productivity of cutting lettuce varieties (Lactuca sativa L. var. secalina) of Dutch breeding in the northern part of the Forest-Steppe zone of Ukraine
Purpose. To investigate the variability of biometrics indices and productivity of cutting lettuce varietis (Lactuca sativa L. var. secalina) of Dutch breeding on soddy medium podzolic soil in the northern part of the For...