Methods to Increase Phenothiazine Conversion in Beauveria bassiana
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2016, Vol 5, Issue 3
Abstract
Aims: The degradation of phenothiazine with the filamentous fungus Beauveria bassiana was studied under different fermentation conditions. The objective was to demonstrate methods to increase substrate conversion in wild type B. bassiana using phenothiazine as a screening substrate. A bio catalytic system was optimized to increase the conversion of phenothiazine by resting cell reactions. Methodology and Results: Reactions were carried out with cells grown in different concentrations of glucose, 5 - 40 g/L, and different resting cell densities, 0.78-6.2 mg cdw/mL. The conversion was monitored with gas chromatography and was characterized by mass spectroscopy, chiral HPLC, and NMR. The highest conversion, 74%±1, was achieved with 0.78 mg cdw/mL. Glucose didn’t have an effect over the conversion of phenothiazine. This fungus oxidized phenothiazine into its sulfoxide and hydroxyl metabolites. Conclusion: B. bassiana degrades more phenothiazine at lower cell densities than higher densities. The success of this project helps us expand the oxidation capacity of B. bassiana as a fungal biocatalyst while improving its utility for industrial purposes.
Authors and Affiliations
Felipe Nicolau, Tonya L. Peeples
Keywords
Related Articles
- EP ID EP351785
- DOI 10.9734/JABB/2016/22703
- Views 104
- Downloads 0
Predicting CD4+ T Lymphocyte Count Using IgG and Total Lymphocyte Count in Newly Diagnosed HIV Infected Patients in Specialist Hospital Sokoto North West Nigeria
CD4+ T cell count and viral load monitoring are expensive and unavailable to most human immunodeficiency virus (HIV) infected people in Africa. There is a need to have another marker of HIV infection that is less resourc...
Elevated Plant Density Effects on Performance and Genetic Parameters Controlling Maize (Zea mays L.) Agronomic Traits
Studying mode of gene action for maize traits under high plant density is a prerequisite for conducting an appropriate breeding program for developing high density tolerant varieties. The objective of this study was to a...
Optimization of Cultural Conditions for Production of Extracellular Polysaccharide by Halomonas xianhensis SUR308 Using Weighted Response Surface Methodology
Optimization of different physico-chemical and cultural parameters such as temperature, pH, incubation time and inoculum dose along with variation of NaCl, glucose and casein hydrolysate concentrations were carried out i...
Assessment of Physico-chemical Parameters of Soils in Fallowing Farmlands on the Abundance of Human Infecting Geohelminths in Mgbuitanwo Emohua, Rivers State, Nigeria
This study was conducted to determine the effects of soil physico-chemical parameters on the abundance of human infecting geohelminths in soils of environments used for open defaecation in Mgbuitanwo Emohua, Rivers State...
Hepatorenal Protective Effects of Pomegranate (Punica granatum) Juice against Nicotine Induced Toxicity in Guinea Pigs
Pomegranate juice possess a marked antioxidant capacity with a high content in tannins, phenols and flavonoids which can directly or indirectly reduce oxidative damage by preventing the excessive generation of free radic...