Microbial Evaluation And Subchronic Toxicity Studies Of Ade & Ade Herbal Drug Formulated For Systemic Detoxification And Diuretic Agent.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 17, Issue 2
Abstract
OBJECTIVE: This study Evaluated Microbial Purity and Sub chronic toxicity studies of Ade and Ade Herbal Drug (AHD) in Wister’s rats. MATERIALS AND METHODS: Microbial purity was evaluated using appropriate diagnostic media. Acute toxicity of the product was evaluated in Swiss albino mice. In Sub chronic toxicity studies, Swiss albino rats weighing 98g-174g were divided into four study groups (n=5 in each cage) and fed with different treatment doses of the formulation for 30days as indicated below: Control group received 0.2ml saline solution Group l received 600mg/kg extract Group ll received 1,200mg/kg extract Group lll received 1,800mg/kg extract At the end of the 30days, the albino rats were sacrificed and blood samples were collected into EDTA and Heparin Bottles respectively for Haematological and Biochemical analyses using standard kits. RESULTS - The LD50 of the product showed no lethality at the highest dose (5,000mg/kg) employed and contained microbial load. Significant (P<0.05) reduction in the RBC values at a dose (600mg/kg and 1,200mg/kg) and significant (P<0.05) increase at a dose (1,800mg/kg) compared with the control groups with reduction in the MCH and MCHC values indicates poor blood formation. There were significant reductions in the TC, HDL- cholesterol, LDL – cholesterol and TG values in all the treated groups compared with the control groups. Therefore, the formulation is beneficiary to cardiovascular risk factors. Significant changes in the weight of the Lung, Heart Liver and kidney but non significant (P>0.05) changes in the values of pancreas compared with the control groups and non significant increase in the AST values in all the doses (600- 1,800mg/kg) but significant (P<0.05) reductions in the ALT values. There was gained in the weight in all the treated rats throughout the study. CONCLUSION- The study revealed that the formulation contained high microbial contaminant which were above acceptable level and would be considered microbiologically unsafe for human consumption. While its sub -chronic toxicity study showed that the formulation reduces cholesterol level at low and high doses in animals, it may be beneficial on cardiovascular risk factor. Consequently, the formulation had no side effect on the liver, heart and the kidney in all the doses, however, the changes observed in all the doses on different physiological parameters, suggests phytochemical screening of the formulation and adherence to Good Manufacturing Procedures(GMPs)
Authors and Affiliations
1Akinbosola Jibayo Philips, Oguntoyinbo Abel,, Kayode Olaniyan
Keywords
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