Micropropagation of an important medicinal plant Catharanthus roseus by using coconut water instead of synthetic plant growth regulators
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2018, Vol 5, Issue 6
Abstract
The present in vitro study was conducted for micropropagation of Catharanthus roseus by using coconut water. The coconut water was used in five different concentrations i.e T1 (3 %), T2 (6 %), T3 (9%), T4 (12 %) and T5 (15 %) v/v in 1L MS medium instead of synthetic plant growth regulators (PGRs). The results showed that in treatment (T4) 80% cultured explant segments showed shoot emergence and the mean number of leaves (4.80) nodes (2.60 ), average shoot length (3.15cm) and % rooting (60%) was also higher followed by the treatment (T3). At lower (T1, T2) and higher (T5) concentration of coconut water the growth of Catharanthus roseus plantlets was suppressed. It has been concluded that the treatment (T4) 12 % coconut water showed the best growth rate of Catharanthus roseus plantlets. The plant growth hormones present in coconut water affected the in vitro microprogation of Catharanthus roseus significantly, therefore, it can be used instead of synthetic plant growth regulators (PGRs) in MS media for micropropagation of Catharanthus roseus.
Authors and Affiliations
Fizza Matloob, Zishan Gul, and Zafar Jamal
Keywords
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