Modulation of antiviral cytokine production and lung protection against influenza virus by the glycosides from Ligustrum purpurascens
Journal Title: Toxicology Communications - Year 2021, Vol 3, Issue 1
Abstract
Background: Ligustrum purpurascens has been used as a traditional herb for over 2,000 years in China. This study was design to investigate the modulation of antiviral cytokines and reduction in lung inflammation of virus-infected mice by the glycosides isolated from Ligustrum purpurascens. Methods: Ligustrum purpurascens glycosides (LPG) were isolated from the leaves of Ligustrum purpurascens. Proliferation of spleen lymphocytes were investigated after LPG treatment. The in vitro and in vivo cytokine modulation of LPG was studied. Furthermore, the anti-inflammatory and antiviral activities of LPG, with the potential to reduce inflammatory lung disorders, were investigated by influenza A virus infected mice. Results: LPG could significantly promote the proliferation, and also could stimulate the production of IFN-γ by spleen lymphocytes in a dose-dependent manner. IFN-γ expression level was increased significantly compared to the control and presented a dose-dependent manner in vitro. Furthermore, LPG inhibit the expression of TNF-α and IL-10, which return to normal level in the cyclophosphamide -induced mice model in vivo. Besides, the histopathological analysis indicated LPG reduced acute lung injury in mice infected with influenza virus. Conclusion: This study suggested that LPG could increase the exression of IFN-γ, immunoregulation and decrease lung inflammation of virus-infected mice.
Authors and Affiliations
Chen-Yang Li, Xiao-Jun Zhong, Xiao-Peng Hu, Qin-Chang Zhu, Hai-Tao Xiao, Peng Zhai, Da-Hong Yao, Muhammad Shahid Riaz, Zhen-Dan He, Xun Song
Keywords
Related Articles
- EP ID EP692821
- DOI 10.12032/ATR20210203
- Views 157
- Downloads 0
Analysis of the metabolic mechanism in Cantharidin-induced hepatotoxicity in LO2 cells using lipidomics analysis
Background: Cantharidin is a major active compound from Banmao (Mylabris). Cantharidin has obvious anticancer activity. However, its clinical application is limited due to serious hepatotoxicity. Methods: To evaluate the...
A study of acupoint selection rules of Moxibustion and Auricular point therapy in the treatment of urinary retention after anorectal surgery based on data mining
Background: Based on the Traditional Chinese Medicine Inheritance Support System (V2.5), we explored the compatibility rules of moxibustion and auricular point prescriptions in the treatment of urinary retention after an...
Identification of signal pathways and biomarkers of plaque psoriasis and prediction of potential microRNA targets via comprehensive strategies
Background: Psoriasis is a complex skin disease and the pathogenesis of psoriasis is not clear. The purpose of this study is to identify the key driving genes and signal pathways involved in psoriasis and to predict the...
Experimental study on acute toxicity of Lucao Wenban granules
Background: To evaluate the acute toxicity of the experienced prescription Lucao Wenban granules on mice and provide a theoretical basis for clinical medication safety. Methods: The mice were not deprived of water for 16...
Association between sleep duration and overweight/obesity among Chinese children and adolescents: a longitudinal study
Background: To determine the associations between sleep duration and overweight/obesity in Chinese children and adolescents. Methods: This longitudinal study analyzed 3,126 children and adolescents aged 7 to 18 years who...