MODULATION OF GONADAL STEROIDS PRODUCTION BY TILAPIA PITUITARY EXTRACT: AN EVALUATION THROUGH IN-VITRO AND IN-VIVO STUDIES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 2
Abstract
Objective: Endocrine regulation strategies are widely used for synchronization of fertility, but in some cichlids species, the treatments are not always effective. This study used tilapia (Oreochromis niloticus) as a model cichlid fish to evaluate homologous pituitary extract in-vitro and in-vivo bioassays.Methods: In this study, guinea pig Leydig cells, tilapia follicular cells, and female tilapia were treated with tilapia pituitary extract (TP) to evaluate the ability of TP to modulate steroid production in-vitro and in-vivo. Sex steroid hormone quantification was performed using enzyme immunoassay (ELISA), and the relative vitellogenin (Vtg) level was measured using Western blot during the maturation cycle of female fish.Results: Treatment with TP in-vitro significantly increased testosterone and estradiol (E2) levels in guinea pig Leydig cells and in tilapia follicular cells, respectively. In-vivo experiments showed a significant increase in plasma E2 and Vtg concentration in the TP-treated female. Interestingly, 40% oocyte maturation was observed in TP-treated adult female tilapia whereas, only 7% was observed in the control group. TP treatment is increased relative fecundity significantly, reaching a production of 15.7±5.8 oocytes/g of female.Conclusion: The outcome of this study suggests that TP has a potential use in the control of cichlid fish reproduction and can be used as an alternative method for fish fry production.Â
Authors and Affiliations
Juan Ortiz Tirado, Daysi MuÑoz, Luis Valladares, Bangeppagari Manjunatha, Rajesh R. Kundapur
Keywords
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