Molecular Characterization of Penicillium Isolates Using Rapd Technique
Journal Title: Annual Research & Review in Biology - Year 2015, Vol 7, Issue 3
Abstract
Molecular analyses were performed on 14 isolates of Penicillium species. The Penicillium isolates examined were collected from different localities and habitats of Jeddah, Saudi Arabia and maintained on Czapek Dox's and Waksman's media. Random Amplified Polymorphic DNA (RAPD-PCR) technique was used in this study to distinguish these isolates. A total of 42 DNA bands were generated by these primers in the Penicillium isolates grown on Czapek Dox's medium while, Penicillium isolates grown on Waksman's medium, 43 DNA bands were generated by the primers. Of the polymorphic bands that were identified in the Penicillium isolates grown on Czapek Dox's medium, two were unique, while no such bands could be detected in the Penicillium isolates grown on Waksman's medium. Penicillium isolates grown on both Czapek Dox's and Waksman's media, 40 monomorphic bands were detected. These monomorphic bands were used to distinguish among the studied Penicillium isolates under study. Data were analysed by a clustering method and similarity coefficients using NTSYSpc version 2.02i. The relationships between the species and isolates are discussed.
Authors and Affiliations
Ehab Abdel-Razik Kamel, Mohamad Elsayed Rashed
Keywords
Related Articles
- EP ID EP353455
- DOI 10.9734/ARRB/2015/13657
- Views 86
- Downloads 0
Pharmacognostic and Wound Healing Studies of the Leaves of Bassia eriophora (Family: Chenopodiaceae) on Albino Rats
Objective: To study the pharmacognostic profile and wound healing effect of aerial parts of Bassia eriophora (Family: Chenopodiaceae), a wild plant in Saudi Arabia, on albino rats. Methods: The microscopy, phytochemical...
Evaluation of the Protective Effect of Acacia senegal Extract against di-(2-ethylhexyl phthalate) Induced Hepato- and Neurotoxicity in Rats
Di-(2-Ethylhexyl phthalate) (DEHP) is an aromatic diester used to improve plasticity of industrial polymers. It exhibited adverse changes in both liver and brain. The current study was planned to evaluate efficiency of A...
A Review of Host-Parasite Relationships
Host parasite relationships result from prolonged associations between organisms living in a given environment. The nature and extent of the association will determine the type of relationship existing between the co-hab...
Green Tea confers Protection on the Retina in MPTP Mice Model of Parkinson’s Disease
Aim: This study investigated the impact of MPTP induced Parkinson’s disease (PD) and the protective and/or curative effects of green tea on the retina. Study Design: Twenty-five adult male mice (Mus musculus) weighing be...
Response to F3 Selection for Grain Yield in Durum Wheat [Triticum turgidum (L.) Thell. ssp. turgidum conv. durum (Desf.) Mac Key] under South Mediterranean Conditions
An experiment was carried out at the Field Crop Institute, Agricultural Research Station of Setif (ITGC-ARS, Setif, Algeria), to assess the response to early generation selection in durum wheat (Triticum durum Desf.). Re...