Molecular Characterization Using gtf Gene Detection and Medium Optimization of Beta-D-Glucan Production from Pediococcus parvulus F1030 Isolated from Local Egyptian Boza
Journal Title: Journal of Pharmaceutical Research International - Year 2017, Vol 20, Issue 5
Abstract
Background: Lactic acid bacteria (LAB) have great benefits which can improve the nutritional value of food, reduces some infections, immune modulator effect, curbs some types of cancer and restricts glucose level of serum. Aims: This study was carried out to detect glycosyltransferase gene (gtf) encoding glucosyltransferase enzyme responsible for beta-D-glucan production in Pediococcus parvulus F1030 which is isolated by authors from local Egyptian Boza (local alcoholic beverage), and enhancing its production by medium optimization. Materials and Methods: Molecular identification and DNA sequencing were performed by using specific primers and the medium optimization was enhanced using Plackett-Burman Design. Results: The isolate detected as Pediococcus parvulus by the molecular identification with using 16S rDNA and submitted in GenBank as Pediococcus parvulus F1030 with accession number ky942131 by the authors. The study revealed the presence of 97% identity with similar to Pediococcus parvulus accession number ky425809. The gtf gene was detected in the isolate by 16S rRNA gene using specific primers. The medium optimization showed enhancement of beta-D-glucan production. The medium number 2 yielded 11 g l-1 from it by using concentration 1.5 g l-1 from sucrose, 0.5 g l-1 from CaCl2, 0.75 g l-1 from tween80, 3 g l-1 from KHPO4 and 1.5 g l-1 from glutamic acid. Conclusion: Molecular characterization of isolated Pediococcus parvulus showed 97% identity with a similar organism in NCBI site, the detection of gtf gene carried out by using PCR technique with specific primers and beta-d-glucan production was enhanced with medium optimization using Plackett-Burman Design.
Authors and Affiliations
Khalid Abd El Ghany, Ragaa A. Hamouda, Hoda A. Mahrous, Sherin M. A. Sharaf, Hanafy A. Hamza
Keywords
Related Articles
- EP ID EP313131
- DOI 10.9734/JPRI/2017/39079
- Views 99
- Downloads 0
Development of a Novel Method for the Analysis of the Drug in Low Concentration Using an Antidiabetic Drug ‘Voglibose’ for the Study
Aim: To develop a novel method for the analysis of the drug in low concentration using an anti diabetic drug ‘voglibose’ for the study. Study Design: Analysis of the drug plays an important role in its development. Assa...
Melatonin Improves Metabolic Abnormalities Induced by HAART in Mice
Aims: Highly Active Antiretroviral Therapy (HAART) is associated with metabolic complications. An unexpected extension of functional implications of melatonin, the neuro-hormone synthetized during the night, has been rep...
Phytochemicals, Antioxidant and Antiproliferative Studies of Some Medicinal Plants from Indian Sub-continent
Aims: The Indian sub-continent has rich culture and a vast botanical diversity with high potential abilities of traditional medicine. However, there is a paucity of information available in scientific literature regardin...
Quality Assessment of Some Brands of Tetanus Toxoids Marketed in Open Markets in South-Eastern Nigeria
Aims: This study was designed to verify the immunogenicity (potency) and the safety of tetanus toxoid vaccines marketed in three large open drug markets in South-Eastern Nigeria. Methodology: Tests for Sterility, formald...
Trimetazidine Prevents Renal Fibrosis Induced by Cyclosporine A in Wister Rats
Cyclosporine A (CyA), an immunosuppressant administered to transplant patients, causes adverse effects such as nephrotoxicity and hepatotoxicity. In kidney slices of rats treated with 25 mg/kg/day of CyA we observed inte...