Molecular detection of Eastern Equine Encephalitis virus in mosquitoes from La Pintada (Antioquia)
Journal Title: Revista MVZ Cordoba - Year 2015, Vol 20, Issue 3
Abstract
Objective. The detection of emerging and re-emerging arboviruses in mosquitoes from urban and rural areas, is fundamental for predict possible epidemic outbreaks in human populations. The Municipality of La Pintada (Antioquia), is characterized by the presence of dry tropical forest relicts, fishing, tourism, farms and mining. An entomological research was performed for explore the possible circulation of arboviruses of public health importance. Materials and methods. Mosquitoes were captured in urban and rural sites in February-April of 2012. The specimens were stored in liquid nitrogens tanks and were grouped using taxonomic keys for genera. RNA extraction from pools and generic/nested RT-PCR was performed for Flavivirus, Alphavirus, Orthobunyavirus (Group Bunyamwera) and Phlebovirus. Results. 1274 mosquitoes were collected, mainly belonging to Culex and Aedes genera. RNA extracts of 64 pools were tested by RT-PCR and one pool was positive for Alphavirus. Sequencing of the RT-PCR product and the analysis with sequences storage in GenBank designate the presence of Eastern equine encephalitis virus (EEEV). Conclusions. This is the first record of natural infection from EEEV in mosquitoes from La Pintada (Antioquia), an area with ecological elements that favor the emergence of emerging and re-emerging arboviruses of medical and veterinarian importance.
Authors and Affiliations
Richard Hoyos L, M. Sc| Universidad de Antioquia, Facultad de Medicina, Grupo de investigación en Medicina Molecular y de Traslación. Carrera 51D Nro. 62-29, Medellín, Colombia.2Universidad Nacional de Colombia – Sede Medellín, Escuela de Biociencias, Grupo de investigación en Sistemática Molecular. Calle 59A Nro. 63-20, Medellín, Colombia. 3Instituto de Salud - Carlos III. Ctra de Majadahonda-Pozuelo Km 2.2, Madrid, España., Juan Suaza V, M. Sc| 2Universidad Nacional de Colombia – Sede Medellín, Escuela de Biociencias, Grupo de investigación en Sistemática Molecular. Calle 59A Nro. 63-20, Medellín, Colombia. 3Instituto de Salud - Carlos III. Ctra de Majadahonda-Pozuelo Km 2.2, Madrid, España., Antonio Tenorio, Ph. D| 2Universidad Nacional de Colombia – Sede Medellín, Escuela de Biociencias, Grupo de investigación en Sistemática Molecular. Calle 59A Nro. 63-20, Medellín, Colombia. 3Instituto de Salud - Carlos III. Ctra de Majadahonda-Pozuelo Km 2.2, Madrid, España., Sandra Uribe, Ph. D| 2Universidad Nacional de Colombia – Sede Medellín, Escuela de Biociencias, Grupo de investigación en Sistemática Molecular. Calle 59A Nro. 63-20, Medellín, Colombia. 3Instituto de Salud - Carlos III. Ctra de Majadahonda-Pozuelo Km 2.2, Madrid, España., Juan Gallego-Gómez, Ph. D *| Universidad de Antioquia, Facultad de Medicina, Grupo de investigación en Medicina Molecular y de Traslación. Carrera 51D Nro. 62-29, Medellín, Colombia.Correspondencia: juanc.gallegomez@gmail.com
Keywords
Related Articles
- EP ID EP3428
- DOI -
- Views 423
- Downloads 18
Fecundity and fertility of Macrobrachium amazonicum (Héller 1862) (Decápoda, Palaemonidae) of Colombian Piedemonte Llanero
Objective. To determine the fecundity and fertility of Macrobrachium amazonicum (freshwater shrimp) of the Piedemonte llanero. Materials and methods. The animals were caught at a fish farm, in the municipality of Restrep...
Follicular dynamics, corpus luteum growth and regression in multiparous buffalo cows and buffalo heifers
Objective. Characterize the follicular dynamics and luteal growth and regression pattern of multiparous (MB) and heifer (BH) Murrah buffaloes in Colombian tropical conditions. Material and methods. Ten MB and ten BH were...
Hematological and clinical chemistry changes induced by acute stress during handling and capture of catfish (Ictalurus punctatus)
Evaluation of hematological and biochemical parameters of culture channel catfish (Ictalurus punctatus) under acute stress by management and capture practice.Fish (200 g mean) were maintained in culture tanks and divided...
Clinical and histopathology evaluation of cutaneous Pythiosis in calves the department of Cordoba, Colombia
Objective. Evaluate and characterize the clinical and histopathological aspects of cutaneous Pythiosis in calves the department of Córdoba, Colombia. Materials and methods. A descriptive, non-probabilistic, convenience...
Trophic ecology of Saccodon dariensis (Pisces: Parodontidae) in Guatapé River tributaries, Magdalena River Basin, Colombia
Objective. To study the trophic ecology of Saccodon dariensis in the Peñoles and El Cardal streams, Guatape River mid-basin, Magdalena River Basin, Colombia. Materials and methods. To compare differences in size between...