MOLECULAR IDENTIFICATION OF PHYTOPLASMA ASSOCIATED WITH SOYBEAN WITCHES-BROOM IN VIDARBHA REGION, MAHARASHTRA
Journal Title: International Journal of Agriculture Sciences - Year 2017, Vol 9, Issue 21
Abstract
Soybean (Glycine max L.) plants showing witches’-broom symptoms typical of phytoplamas were observed from different locations of Vidarbha region, Maharashtra. Symptoms observed under field condition include phyllody, stunting growth, proliferation of auxiliary shoots resulting in a witches-broom appearance and deformed pods if any. PCR detection of soybean phytoplasma by using phytoplasma-specific universal primer pairs (P1/P7) produced an amplified product of 1.8kb lengths when DNA extract from symptomatic samples was used as template. Asymptomatic plant samples yielded no amplification. On mechanical inoculation none of the test plants inoculated showed any visible symptoms even after 120 days after inoculation
Authors and Affiliations
M. HAJONG, P. C. ATRAM, S. S. MANE
Keywords
Related Articles
- EP ID EP172492
- DOI -
- Views 142
- Downloads 0
SCALE TO MEASURE THE UTILITY PERCEPTION OF CATTLE OWNERS
The term utility perception about cattle by the owners has been a problem for a researcher in agriculture. Keeping in view the subjectivity, there was a need to evolve a methodology, which would introduce much needed obj...
GENETIC VARIABILITY ESTIMATES OF QUANTITATIVE AND QUALITATIVE TRAITS IN BLACK GRAM
Black gram (Vigna mungo L.) has the potential of supplying a major portion of dietary protein demand and restoring the soil health at the same time. Research on genetic variability studies of the genotypes may increase t...
AFMR (AGRICULTURAL FM RADIO): A TOOL FOR TRANSFORMING AGRICULTURAL INFORMATION IN RELATION TO DIFFERENT VARIABLES OF EXTENSION EDUCATIONISTS
To know the personal and social variables of the extension educationists for transferring agricultural information through Agricultural FM Radio (AFMR), a study was conducted on a random sample of 60 extension educationi...
BIOMONITORING OF SEED MYCOFLORA CONTAMINATION OF FRESHLY HARVESTED IN MAIZE GROWING ZONE-I
Maize is of the main cereal produced. In this study the seed mycoflora of freshly harvested maize of zone-I were isolated by standard technique i.e. Agar plate method (APM) and Blotter plate method (BPM). A total of 9 ge...
EVALUATION OF CHICKEN CUTLETS INCORPORATED WITH WHOLE EGG LIQUID
The present study was conducted to the effect of different levels viz. 0% (T1), 2.5% (T2), 5% (T3) and 7.5% (T4) of whole egg liquid incorporation on physico-chemical and sensory parameters of chicken cutlets with its co...