Molecular Typing Of Capsular Polysaccharides of Staphylococcus Aureus Isolated From Cases of Bovine Mastitis by PCR
Journal Title: Concepts of Dairy & Veterinary Sciences - Year 2018, Vol 1, Issue 1
Abstract
Forty five Staphylococcus aureus isolated from cases of bovine mastitis were subjected to Molecular typing by Polymerase chain reaction to determine their capsular polysaccaharide type. Of the 45 isolates, 33 were confirmed to carry a cap5 locus and cap8 locus was detected in remaining 12 isolates. To the best of our knowledge this is the first report of capsular polysaccharide typing of S.aureus isolates from India. S. aureus produces a variety of extracellular and cell wall associated components which are involved in the pathogenesis of bovine, ovine and caprine mastitis [1]. S. aureus strains produce capsular polysaccharide (CP) in-vivo [2] or under defined culture conditions [3]. Although capsule production of staphylococci was first recognized in 1930 [4], the prevalence of encapsulation among S. aureus has been appreciated only recently. Eleven capsular polysaccharide serotypes have been proposed on the basis of agglutinating reactivity with adsorbed rabbit antiserum and precipitation in double immuno diffusion [5,6]. Of these capsular serotypes 5 and 8 are the most predominant serotypes in human and animal S. aureus infections.
Authors and Affiliations
Krithiga N, Antony PX, Tollersrud T, Mukhopadhyay HK, Pillai RM, Vijayalakshmi P, Thanislass J
Keywords
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- EP ID EP590862
- DOI 10.32474/CDVS.2018.01.000102
- Views 111
- Downloads 0
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Molecular Typing Of Capsular Polysaccharides of Staphylococcus Aureus Isolated From Cases of Bovine Mastitis by PCR
Forty five Staphylococcus aureus isolated from cases of bovine mastitis were subjected to Molecular typing by Polymerase chain reaction to determine their capsular polysaccaharide type. Of the 45 isolates, 33 were confir...