Morphological changes in various organs of rats in cases ofalloxan-induced diabetes
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 30, Issue
Abstract
Introduction. Diabetes mellitus (DM) type 1 (or insulin-dependent DM) is a chronic endocrine and metabolic disease due to an absolute lack of insulin because of the combined effect of various endogenous (genetic) and exogenous factors that cause violation of all kinds of metabolism, vascular disease, nerves, different organs and tissues. However, the issue of etiology and pathogenesis of diabetes mellitus in animals is understudied, and effective treatment methods of this disease are actually absent. That is why these issues remain open and relevant even nowadays. The goal of the work is to study morphological changes in organs of the experimental rats organs (liver, pancreas, heart, kidneys) with the experimental alloxan-induced diabetes mellitus. Materials and methods. Female rats aged 4-5 months with an average weight of 230 g were used in the research. Experimental model of diabetes mellitus was reproduced by a single subcutaneous injection of alloxan monohydrate in a dose of 150 mg/kg in the form of 5% solution on citrate buffer (pH 4,5) after 24 hours of preliminary absolute diet (with free access to water). To reduce animal deaths caused by hypoglycemic shock after diabetes induction rats received 5% glucose solution instead of water. Tissue sampling for histological research during the study of changes in alloxan-induced diabetes mellitus was conducted on day 20. To examine the tissue microstructure, cross sections were studied with hematoxylin and eosin to detect connective tissue using Van Gieson's stain. Results of research and discussion. The research of the morphological changes in pancreas, liver, kidneys and heart at alloxan-induced diabetes mellitus was conducted. It was foundthat this model of diabetes mellitus is accompanied by structural changes in the endocrine part of the pancreas, which depend on the disease severity. At the same time, no visible histological changes of exocrine part were found. In the liver there were signs of chronic hepatitis with hepatoportal necrosis and fibrosis, as well as congestions in blood vessels. Signs of glycogen nephrosis and numerous composites of calcium salts were noted in the observed samples of kidneys. The signs of myocardosis and endocardium thickening were found in the heart. Conclusion and prospects for further research. Revealed morphological changes in the pancreas, liver, kidneys and heart at experimental alloxan-induced diabetes mellitus are characteristic for the pathogenesis of spontaneous diabetes. Described in the article data is relevant, since it enables further research of various treatment methods on the presented model of diabetes mellitus.
Authors and Affiliations
Anatoly Mazurkevich, Vitaliy Kovpak, O. Kovpak, Natalia Hudz
Keywords
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- EP ID EP271578
- DOI 10.31073/vet_biotech30-20
- Views 130
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