Morphometric Assessment of Chronic Inflammatory Cells in Colonic Biopsies of Patients with Irritable Bowel Syndrome
Journal Title: Journal of Clinical and Diagnostic Research - Year 2019, Vol 13, Issue 5
Abstract
ABSTRACT Introduction: Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder which has a complex pathophysiology including the role of inflammation being recently studied. Aim: To assess the role of mast cells and Intraepithelial Lymphocytes (IELs) in IBS patients by morphometry. Materials and Methods: In this study, 148 cases of IBS using the Rome III criteria and 28 controls were taken. IBS patients were divided into two subgroups of Post Infectious (PI) and Non Post Infectious (NPI) subgroups on the basis of past history of episodes of gastroenteritis. All of them underwent full length colonoscopy and biopsy from the descending colon. Histopathology of these two were compared with special attention to morphometric count of IELs by “pin hole” method and mast cells using toluidine blue highlighting. Results: Biopsies were unremarkable in 23(15.54%) patients, whereas 125 (84.46%) patients were diagnosed as Non Specific Colitis (NSC). The difference in IEL counts and mast cell counts were extremely significant (p<0.0001) in controls vs patients, controls vs PI-IBS patients and PI-IBS vs NPI-IBS subgroup whereas the difference was significant (p<0.001) in controls vs. NPI-IBS subgroup. Conclusion: The present study concluded that mast cells and IELs show a significant difference between IBS and non IBS cases, and could have an important role to play in the pathophysiology of IBS.
Authors and Affiliations
Anshul Singh, Anupriya Nautiyal, Shailesh R Patel, Vatsala Misra, SP Misra, Manisha Dwivedi
Keywords
Related Articles
- EP ID EP586010
- DOI 10.7860/JCDR/2019/40569.12852
- Views 100
- Downloads 0
Cytodiagnosis of Extraoral Plasmablastic Lymphoma in an HIV-positive Paediatric Patient- A Case Report and Review of the Literature
ABSTRACT Plasmablastic Lymphoma (PBL) is a rare and aggressive form of Non-Hodgkin Lymphoma (NHL), initially described in the oral cavity of Human Immunodeficiency Virus (HIV) positive individuals. Recently, it has been...
Bite Force Recording Devices - A Review
In dental research, bite force serves as a valuable parameter to evaluate the efficacy of masticatory system. A variety of devices with different design and working principle have been used to record bite force, but no s...
Concurrent Acute Glomerulonephritis and Retropharyngeal Abscess in 10 Year Boy: A Case Report
Postinfectious Glomerulonephritis (PIGN) usually follows 1-2 weeks after respiratory tract infection and 4-6 weeks after skin infection. Acute Glomerulonephritis (AGN) is uncommon with simultaneous severe throat infectio...
Estimation of Serum Butyryl Cholinesterase in Patients with Oral Squamous Cell Carcinoma: A Cross-Sectional Study
Introduction: Oral cancer is a major global threat to public health. It is one of the most common causes of mortality and morbidity in the modern era. Oral Squamous Cell Carcinoma (OSCC) accounts for over 90% of the mali...
Co-existence of Wuchereria bancrofti Microfilaraemia in a Case of Multiple Myeloma: An Incidental Finding or an Association?
Filariasis is a global health problem which is endemic in tropical and subtropical regions of Asia, Central and South America and Pacific Island nations. The most common parasite causing lymphatic filariasis in India is...