Multidrug Resistance, V16S rRNA Gene Sequencing, High Frequency of Toxins Encoding Genes (tox, tdh, trh) in Parahaemolyticus and Non -parahaemolyticus Vibrios Isolated from Shellfish
Journal Title: Journal of Advances in Microbiology - Year 2017, Vol 6, Issue 1
Abstract
PCR amplification of V16S-rRNA, tox, tdh, trh was applied as a molecular typing approach to identify potentially toxigenic vibrio species. Thirty vibrio strains were isolated from several types of shellfish collected from Ismailia district, Egypt. Isolates were recognized by conventional biochemical features and PCR amplification then tested for their sensitivity against twelve antibiotics. Multiple sequences alignment of V16S-rRNA indicated the high incidence of V.parahaemolyticus (n= 6) V. cholerae (n=5) and V. fluvialis (n=5) among the investigated groups. V. harveyi (n=3), V. alginolyticus ( n=2 )V. vulnificus (n=1) and (n=8) vibrio sp were reported as well. Toxins encoding genes (tox, tdh, trh) shown high incidence frequency in the majority of the identified vibrio species. Identified isolates were reported as potential pathogenic and multiple antibiotic resistant with noticeably high resistant indexes ranged from (0.5-1). The results significantly emphasized that the isolates have been originated from potential risk sources of infection.
Authors and Affiliations
Amira Mohamed Zakaria, Dalia Mohamed Sabri
Keywords
Related Articles
- EP ID EP338214
- DOI 10.9734/JAMB/2017/36875
- Views 79
- Downloads 0
Infective Keratitis in Alexandria Main University Hospital
Background: Blindness is and, apparently always has been, a problem in Egypt. Corneal blindness is a major public health problem in which; 1.5–2.0 million new cases of monocular blindness reported annually in developing...
Evaluation of the Xpert® MTB/RIF Assay and Microscopy for Diagnosing Pulmonary Tuberculosis in Butembo, Democratic Republic of the Congo
Objective: This study aimed to evaluate the efficacy of Xpert® MTB/RIF assay comparing to the acid-fast smear microscopy for diagnosing pulmonary tuberculosis cases in Butembo, Democratic Republic of the Congo. Methodolo...
Phytochemical and Antimicrobial Activity Screening of Ether Extract of Afromomum melegueta Seed
Aim: The search for new antimicrobials as a result of antibiotic failure has led to the development of new antibiotic scaffolds. In the African sub-continent, this has translated to exploration of her rich flora for pote...
Antimicrobial Effects of Garlic Extracts against Multidrug Resistant Staphylococcus aureus Isolates from Clinical Specimens
Aim: To assess the antibacterial potentials of Allium sativum and their interaction with antibiotics against clinical isolates of multidrug resistant Staphylococcus aureus. Study Design: The study is designed to investi...
Microbiological Analyses of Kunu Drinks Locally Produced and Sold in Calabar, Southern Nigeria
Kunu is a non-alcoholic Nigerian beverage produced locally from cereals. The method of preparation, as well as the key ingredients and spices added as flavoring agents are always not standardized. This study was carried...