Multidrug Resistant Providencia stuartii in Chicken Droppings: Public Health Implications for Poultry Workers and Associated Communities in nearby Dhaka Metropolis, Bangladesh
Journal Title: Microbiology Research Journal International - Year 2016, Vol 14, Issue 5
Abstract
Introduction: Poultry farms (PFs) have appeared successful and wide spread business-industry in Bangladesh, which often remains contaminated with various hazardous microorganisms when standard hygiene practices are compromised. We sought to investigate a zoonotic human pathogen, Providencia stuartii and their antibiotic resistance pattern in chicken droppings collected from local poultry farms in Savar area, Dhaka, Bangladesh. Materials and Methods: We conducted a cross-sectional study to find the prevalence of antibiotic resistance in bacteria from chicken droppings in linked to antibiotic-uses and abuses in PFs. Random chicken droppings were collected from broiler type chickens, layer-chickens, and pre-starter broiler chickens to make samples representative. Following standard bacteriological culture, semisolid chicken-droppings were diluted aseptically, enriched in buffered peptone water, and then streaked onto a xylose-lysine-deoxycholate agar plate. Selected P. stuartii colonies were identified biochemically using API 20E (BioMe´rieux) and antimicrobial susceptibility testing was performed following Kirby-Bauer (disk-diffusion) method. Results: We reported the isolation of P. stuartii for the first time in Bangladesh in chicken droppings collected from randomly selected local PFs. Red colored colonies without black centre on XLD medium were considered as presumptive Providencia stuartii that were subsequently confirmed by API 20E system. Six chicken-droppings revealed the presence of P. stuartii from a total 70 samples tested, showed a prevalence of 8.6% with overall farm prevalence is 71.4%. We took 11 isolates from the six positive samples to examine their antibiotic resistance and found 82% of them were resistant to nalidixic, 73% to ampicillin, and 54.5% to trimethoprim- sulfamethoxazole. Relatively ciprofloxacin and gentamicin appeared more functional, where only 27.3% and 18.2% strains showed resistant, respectively. Over 54% of the isolates appeared resistant to >3 antibiotics and 36.4% with two different antibiotics. None of the isolates remained susceptible to all the 6 antibiotics tested. Conclusion: Detected MDR P. stuartii in chicken-droppings from local poultry farms may contribute their transmission to surrounding communities and may implicate serious biosecurity concern in environmental and food-safety issues in resource constraint countries, like, Bangladesh.
Authors and Affiliations
Arifatun Nahar, Mahfuza Marzan, Mashuk Siddiquee, Shamsun Nahar, Kazi Selim Anwar, Salequl Islam
Keywords
Related Articles
- EP ID EP350900
- DOI 10.9734/BMRJ/2016/25883
- Views 96
- Downloads 0
Optimization of Cultural Parameters for Cost Effective Production of Kojic Acid by Fungal species Isolated from Soil
Two different species of Aspergillus are able to produce kojic acid at higher concentrations under static conditions in the present study. Various physicochemical parameters which influence the production of kojic acid a...
Antibiotics Susceptibility Profile of Listeria species Isolated from Untreated Abattoir Wastewater in Akinyele, Ibadan, Nigeria and Its Implication on Public Health
Introduction: The demand for meat to meet the need of increasing human population has led to increase in the abattoir activities. Untreated abattoir wastewaters which may constitute a public health threat due to the poss...
Antimicrobial Potential of Dacryodes edulis against Selected Clinical Bacterial Isolates
The antimicrobial potential of Dacryodes edulis was investigated to determine the antimicrobial properties of the collected raw D. edulis pulp and seed extracts on some medically important human pathogens. The research w...
Association of Hepatitis Virus Infections with CD4 and CD8 among Certain Group of Lymphoma Patients in Babylon Provence
This study was planned to detect the immunological association impact of hepatitis infection in lymphoma patients, There are two branches of this study, firstly theoretical background and secondly is practical as diagnos...
Evaluation of Three Phenotypic Tests vs. Duplex (coa & mecA) PCR for Detection of Methicillin-Resistance Staphylococcus aureus (MRSA) Strains from Patients with Nosocomial Infections (NI) in a Mexican Hospital
Background: Staphylococcus aureus (SA) is a leading cause of hospital acquired infections worldwide. Epidemiological features have also changed in Mexican hospitals and methicillin-resistance Staphylococcus aureus (MRSA)...