Multiplex PCR for molecular screening of Borrelia burgdorferi sensu lato, Anaplasma spp. and Babesia spp.
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2015, Vol 22, Issue 4
Abstract
[b]Introduction.[/b] Ticks transmit a great variety of pathogenic microorganisms to humans and animals. The detection of tick-borne pathogens (TBP) is mainly by molecular techniques based on polymerase chain reactions (PCR). [b]Objective.[/b] To design and evaluate a multiplex PCR for the molecular screening of zoonotic TBP for exploratory studies. [b]Material and methods[/b]. Control DNA from reference strains, DNA from experimentally-infected biological specimens, and from [i]Rhipicephalus sanguineus[/i] ticks collected from domestic and homeless dogs were used. A multiplex PCR assay to detect the presence of [i]Borrelia burgdorferi[/i] sensu lato, [i]Anaplasma[/i] spp. and [i]Babesia[/i] spp. was designed and optimized using primers previously reported for [i]B. burgdorferi[/i] sensu lato and [i]Anaplasma[/i] spp., while for[i] Babesia[/i] spp. they were designed in silico. The multiplex PCR was evaluated on the DNA from biological samples. [b]Results[/b]. A new set of specific primers for Babesia spp. was designed. Adjustment of the master mix reactive concentrations and amplification conditions for the multiplex PCR allowed the successful amplification of the specific amplicons for each microbial group from the control DNA and experimentally-infected biological specimens. The efficiency of the multiplex PCR amplifying three DNA targets was confirmed. Individual and co-infection of [i]Anaplasma [/i]spp. and Babesia spp. were detected in the [i]R. sanguineus[/i] ticks from dogs. [b]Conclusions[/b]. A multiplex PCR assay for the screening of three TBP is available. By using it, B. burgdorferi sensu lato, [i]Anaplasma[/i] spp. and Babesia spp. can be detected accurately in one PCR reaction.
Authors and Affiliations
Islay Rodríguez, Caroline Burri, Angel Noda, Véronique Douet, Lise Gern
Keywords
Related Articles
- EP ID EP81026
- DOI -
- Views 160
- Downloads 0
Survey of pesticide application on vegetables in the Littoral area of Togo
Vegetable production in Togo is seriously affected by pests attack. To reduce damage, farmers indiscriminately use pesticides. Various studies have reported high concentrations of pesticide residues more than acceptable...
Can thyroid size still be considered as a useful tool for assessing iodine intake?
Introduction. It has always been very difficult to precisely define a goitre. For years, the borderline values have been sought which could be universally used in such evaluations. However, presented reference values wer...
Ekspozycja na kleszcze oraz seroprewalencja Borrelia burgdorferi wśród zdrowych młodych osób zamieszkałych na terenie południowego Podlasia (Polska)
Celem badań była ocena ryzyka zakażenia Borrelia burgdorferi na terenie południowego Podlasia (przy granicy z Białorusią) poprzez analizę postępowania poekspozycyjnego oraz ocenę bezobjawowego zakażenia młodzieży ukłutej...
Life style and risk of development of breast and ovarian cancer
Introduction: Many risk factors may be monitored, and individual decisions concerning life style exert an effect on most factors associated with the development of cancer. It is estimated that the majority of malignant c...
Co-existence of chronic non-communicable diseases and common neoplasms among 2,462 endocrine adult inpatients – a retrospective analysis
[b]Objective[/b]. To analyze the coexistence of chronic non-communicable diseases (NCDs) and common neoplasms among endocrine adult inpatients. [b]Materials and method. [/b]The retrospective analysis was performed usin...