Antimicrobial potential of Halophilic actinomycetes against multi drug resistant (MDR) ventilator associated pneumonia causing bacterial pathogens
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2016, Vol 29, Issue 2
Abstract
A collection of forty halophilic actinomycetes isolated from water and mud samples of the saline lake at Kalar Kahar, salt range, Pakistan, was screened to investigate their antimicrobial potential against multi drug resistant (MDR) ventilator associated pneumonia causing bacterial pathogens. The isolates exhibited significant tolerance to alkaline conditions and grew well at pH 9-11. The taxonomic status of the isolated strains was determined by morphological, biochemical and physiological characterization and by 16s rRNA gene sequencing. The results revealed that majority of the isolates (90%) belong to the genus Streptomyces. Most of the isolates exhibited remarkable antimicrobial activity up to 20mm zone of inhibition against MDR ventilator associated pneumonia causing bacteria including Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, Escherichia coli, Enterobacter and Acinetobacter spp. Additionally the isolates showed moderate to high cytotoxicity in the range of 40 to 80% larval mortality against Artemia salina in a micro well cytotoxicity assay. The chemical screening or the so called metabolic fingerprinting of the methanolic extracts of each isolate, by thin layer chromatography (TLC) using various staining reagents and by high performance liquid chromatography (HPLC-UV), indicated an impressive diversity of the compounds produced by these strains. The study reveals that these halophilic actinomycetes are a promising source of bioactive compounds. The preparative scale fermentation, isolation, purification and structure elucidation of the compounds produced by them may yield novel antimicrobial or chemotherapeutic agents.
Authors and Affiliations
Sana Aslam , Imran Sajid
Keywords
Related Articles
- EP ID EP123131
- DOI -
- Views 124
- Downloads 0
Validated spectrofluorimetric method for determination of sulpiride in commercial formulations using Hantzsch condensation reaction
A simple, sensitive, selective and cost effective spectrofluorimetric method has been established for the quantification of sulpiride after their complete alkaline hydrolysis. The method is based on the condensatio...
Neuroprotective effects of electro acupuncture on hypoxic-ischemic encephalopathy in newborn rats Ass
[sub][/sub] Hypoxic-ischemic encephalopathy (HIE) is a common and potentially devastating condition in the neonate, associated with high mortality and morbidity. Effective treatment options are limited and therefore...
Hypolipidemic effect of aqueous extract of Carum carvi (black Zeera) seeds in diet induced hyperlipidemic rats
Medicinal plants play a key role in preventing various diseases. Hyperlipidemia is a major contributor to the pathogenesis of cardiovascular diseases. The purpose of the present study was to assess the effect of aqueous...
Densitometric HPTLC method for qualitative, quantitative analysis and stability study of coenzyme Q10 in Pharmaceutical formulations utilizing normal and reversed-phase silica gel plates
Two simple, preciseand stability-indicating densitometric HPTLC method were developed and validated for qualitative and quantitative analysis of Coenzyme Q10in pharmaceutical formulations using normal-phase (Method...
Hypocholestrolic effect of spent black tea leaves replaced with wheat bran in broiler ration Attaur
Black tea leaves (Camellia sinensis) have been known for many years in lowering cholesterol level. The purpose of the present study was to find the effects of spent black tea leaves as a substitute of wheat bran on...