B-Cell Non-Hodgkin’s Lymphoma characterisation by immunohistochemistry in Indigenous black Zambians
Journal Title: Journal of Medical Science and Technology - Year 2015, Vol 4, Issue 1
Abstract
B-cell Non-Hodgkin’s lymphomas are a heterogeneous disease entity, accounting for about 21 different subtypes and 85% of all NHLs. This heterogeneity often makes clinical management of these patients difficult. Currently very little is known about the immunophenotype of the B-cell NHLs in Zambia. Immunohistochemistry plays a crucial role in the proper classification, determination of prognosis and an optimum therapeutic schedule in these lymphoid malignancies. This study was aimed at characterizing the immunohistochemical expression of 5 proteins (CD10, BCL-2, BCL-6, CD23 and Cyclin D1) in 28 cases of B-cell NHLs. This was a cross-sectional study conducted to evaluate 28 conveniently sampled B-cell NHLs formalin fixed paraffin embedded tissues from the year 2012 at the University Teaching Hospital for a six month period. The following panels of primary antibodies were used: CD10, CD23, CyclinD1, BCL-2 and BCL-6 (Dako, Glostrup, Denmark). The Labelled Streptavidin Binding (LSAB) staining was used to amplify and view the reaction. Fisher’s exact test was used to indicate statistical significance of the findings. The B-cell NHLs showed positive expression to the markers as follows; Diffuse large B cell lymphoma CD10 3/11(27.4%), BCL-2 6/11(54%) and BCL-6 1/11(9.1%). Burkitt lymphoma, CD 10 4/5(80%) and BCL-6 4/5 (80%). Small lymphocytic lymphoma, CD10 1/2(50%), BCL-2 1/2(50%) and, CD 23 2/2(100%). Mantle cell lymphoma CD10 ¼(25%), BCL-2 3/4(75%) and Cyclin D1 4/4(100%). Follicular lymphoma was CD10 1/2(50%). About 4 cases (14.3%) of the B-cell NHL were unclassified and they were positive only for CD10 3/4(75%). The B-cell NHLs has shown variable expression of the surface membrane proteins. Specifically the over expression of BCL-2 and reduced expression of CD10 and BCL-6 by DLBCLwhich accounted for the majority of the B-cell NHLs is suggestive of a very aggressive NHL prevailing among indigenous black Zambians.
Authors and Affiliations
Marah Simakando, Trevor Kaile, Lidia Korolova, Evans Malyangu, Aaron Shibemba
Keywords
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