DEVELOPMENT OF A STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF TRIMETHOPRIM, SULFAMETHOXAZOLE AND METYHL PARABEN IN ORAL SUSPENSION
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 2
Abstract
ABSTRACT A gradient Simultaneous estimation by RP-HPLC Method were developed and validated for the quantification of Trimethoprim, Sulfamethoxazole and methyl paraben at single wavelength (275nm) in order to assess assay and in vitro drug release profile of drug from Oral Suspension formulation. A gradient elution of samples performed on Lichrospher 60-RP select B, 5µm, Lichrocart MERCK (250 × 4.0 mm, 5μm) with buffered mobile phase consisting solvent A of KH2PO4 0.05M , solvent B (Acetonitrile) and solvent C (Methanol) in ratio of (75:15:10) (v/v/v) delivered at flow rate 1.0 mL/min. The average retention time for Trimethoprim, Sulfamethoxazole and methyl paraben were found to be 6.4 min, 16.8 min and 26.6 min. The proposed method was validated for selectivity, precision, linearity and accuracy. The assay methods were found to be linear from 140-260 µg/ml for Sulfamethoxazole, 28-52µg/ml for Trimethoprim and 7.0-13.0µg/ml for methyl paraben. In addition filter suitability, standard and sample solution stability was demonstrated. The validated method is suitable for quality control applications and its advantages over the already existing methods are simplicity and reduced analysis time. Keywords: RP-HPLC Method; Simultaneous Estimation; Stability; Sulfamethoxazole; Trimethoprim.
Authors and Affiliations
Lazar M * and Mouzdahir A
Keywords
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- EP ID EP133504
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