Effect of Thermal Stress on Caprine Hepatocyte’s Antioxidant Enzymes Activity and TGF β Secretion In Vitro
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2014, Vol 10, Issue 2
Abstract
Liver is involved in several vital functions such as synthesis, secretion, storage and metabolism of plasma proteins and in detoxifying functions. In the present study, hepatocytes were obtained by disaggregation of caprine liver caudate lobe. The cells were cultured under controlled conditions in a CO2 incubator at different temperatures. The function of hepatocyte culture was evaluated in terms of secretion of albumin, urea and acitivity of Lactate dehydrogenase. Cells were cultured at three different temperatures, i.e. 37, 40 and 42o C, and at each temperature, the culture was propagated for 24,48 and 72h time interval respectively. At 37oC, the concentration of albumin and urea increased in the culture temporally where as activity of LDH decreased by 72h. The least square mean (LSM) concentration of albumin, urea in culture decreased and activity of LDH increased significantly (P<0.01) at 40 and 42oC, when compared with respective values at 37oC. Hence effect of thermal stress(40 and 42oC) on activity of hepatocyte antioxidant enzymes ( super oxide dismutase, catalase and glutathione peroxidase) and TGF β secretion in vitro was further evaluated. It was observed that there was significant increase (P<0.01) in the activity of the antioxidant enzymes . The LSM activity of all the enzymes increased significantly at 40 and 42oC when compared with the enzyme activity at 37oC, but increase in the secretion of TGF β could be observed only at 42oC post 48h of incubation. TGF β is associated with apoptosis and cytotoxicity. It was observed that viability of the cells decreased significantly (P<0.05) only at 42oC post 48h of incubation. It can be concluded that hyperthermic conditions were observed to be stressful for hepatocytes during in vitro conditions, which proved to be more significant at 42oC post 48h of incubation. Further work has to be carried out with some supplements which can reduce the hyperthermic effect. In vivo studies in caprine species will give further insight on thermal stress. To the best of our knowledge no studies on hyperthermic effect on hepatocytes in caprine species in vitro or in vivo has been reported.
Authors and Affiliations
Phad Vyankati, Anand Laxmi
Keywords
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