Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time
Journal Title: RSBO - Year 2012, Vol 9, Issue 1
Abstract
Introduction and objective: The main exogenous factors limiting the retrieval of information from human remains are fire and accidents involving high temperatures. Teeth, due to their relatively high degree of chemical and physical resistance, offer the possibility for the recovery of genetic material, becoming important in forensic cases. With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. Material and methods: Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. Results and conclusion: After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed.
Authors and Affiliations
Ricardo Silva, Rodrigo Quiezi, Claudia Bertolacini, Suzana Carvalho, Kellen Gasque, Carina Silva, Lucilene Bicudo
Keywords
Related Articles
- EP ID EP155784
- DOI -
- Views 215
- Downloads 0
Undergraduates’ opinion after 5-year experience with rotary endodontic instruments
Introduction: Dentistry evolution in the past few years has revolutionized daily practice in some specialties. One of these revolutions has occurred in Endodontics due to the advancement of rotary techniques for root can...
A utilização do vidro bioativo na terapia regenerativa periodontal - uma revisão da literatura
Introduction and objective: The correction of the osseous defects caused by periodontal disease is performed through surgical procedures that are divided as resective, reparative or regenerative. The purpose of this pape...
Importance of the information provided to patient before, during, and after endodontic treatment considering the Consumer’s Defense Code
Introduction: The Consumer’s Defense Code was published twenty-years ago and must be properly understood by all service providers, including those of health care area. In this code, the rights and obligations established...
Quality of periapical radiographs taken by undergraduate students during endodontic treatment
[b][/b]Introduction: Correct radiographic interpretation depends on the availability of high quality images, free from technique and processing errors. Documenting the kind and the frequency of errors, as an integral par...
Initial endosseous healing response to lactide/glycolide copolymer
Introduction: The search for less invasive treatments, with fast and effective bone regeneration, has lead to the development of synthetic bioresorbable alternatives for bone graft. The commercial product FisiograftTM ge...