Hydnophytum formicarum Jack ethanol extract modulates quorum sensing-controlled pathogenicity in Pseudomonas aeruginosa
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 5
Abstract
The discovery of new mechanism to control microbial pathogenicity by quorum sensing modulation has generated the search for quorum sensing inhibitor from natural resources. The objective of this research was to evaluate the ability of Hydnophytum formicarum Jack (Rubiaceae) ethanol extract to antagonize cell-to cell communication. Pulverized H. formicarum tuber was macerated in ethyl alcohol 96% and evaporated to yield ethanol extract. A dillution technique using Luria-Bertani (LB) medium was used to observe the capability of the extract to reduce the violacein production in Chromobacterium violaceum. Samples in two-fold dilution were prepared to obtain 2 - 0.0625 mg/mL concentration. The effects on swimming, swarming and twitching motility as well as the formation of biofilm towards Pseudomonas aeruginosa PAO1 were recorded over control. All experiments were done in triplicate. The architecture of Ps. aeruginosa biofilm treated with samples was examined by CLSM (Confocal Laser Scanning Microscopy) . Our results suggested that the ethanol extract of H. formicarum caused violacein production inhibition. Furthermore, inhibition of Ps. aeruginosa motility and biofilm formation were recorded to be significant over control in a concentration dependant manner. H. formicarum serves as a potential source for new QS-based antibacterial drugs towards Ps. aeruginosa.
Authors and Affiliations
Triana Hertiani , Sylvia Utami Tunjung Pratiwi
Keywords
Related Articles
- EP ID EP95355
- DOI -
- Views 120
- Downloads 0
Antimicrobial potentials of different solvent extracted samples from Physalis ixocarpa
The present study investigates the antimicrobial activities of different solvent extracted samples isolated from different parts of Physalis ixocarpa through disc diffusion assay using three different concentration...
Synthesis and antibacterial screening of S-substituted derivatives of 5-(3,4-methylenedioxyphenyl)-1,3,4-oxadiazol-2-thiol
The most emerging class among the heterocyclic compounds is 1,3,4-oxadiazoles for their diverse biological activities. In the present research work, piperonylic acid (1) was converted consecutively into correspondi...
Preliminary phytochemical screening, antimicrobial and antioxidant activities of Euphorbia milli
Euphorbia milii is a Pakistani herb used for various infectious diseases. In this study we have carried out phytochemical, antibacterial and antioxidant investigation of different extracts/fractions. Phytochemical...
The possible counteractive effect of gold nanoparticles against streptozotocin-induced type 1 diabetes in young male albino rats
The current study was performed to study the effect of biologically synthesised gold nanoparticles (AuNPs) to control hyperglycaemic conditions in streptozotocin (STZ)-induced diabetic mice. In this study, the rats...
The establish of the HPLC method to examine the plasma concentration of lamotrigine and oxcarbazepine
To establish the HPLC method to examine plasma concentration of lamotrigine and oxcarbazepine. This study set chlorzoxazone as the internal standard, chromatographic column was Column C18 (200×4.6mm, 5um) of DIKMA...