In vitro antioxidant effect of Camellia sinensis on human cell cultures
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 5
Abstract
Camellia sinensisis traditionally used in many polyherbal preparations for the treatment of different diseases and infections. Its action has been associated with its antioxidant activities. In this study, antioxidant effect of Camellia sinensis on hydrogen peroxide-induced human lymphocyte cell cultures was estimated. Camellia sinensis showed high contents of ascorbic acid, phenols, flavonoids, and flavonols. Good scavenging activity was evident by scavenging assays e.g. 2,2-DiPhenyl-2-Picrylhydrazyl Hydrate (DPPH), 2,2-Azinobis (3-ethyl-BenzoThiazoline-6-Sulfonic acid (ABTS) radical assay and reducing power assay. Moreover, High Performance Liquid Chromatography (HPLC-UV) chromatographs showed many notable peaks of unidentified bioactive compounds. In vitro antioxidant actions were determined by the activities of catalase (ELISA kit method), superoxide dismutase, lipid peroxidation and total protein contents on lymphocyte cell cultures. In vitro experimental trial showed strong antioxidant repair mechanism of plant against oxidative stress. Results of extraction with solvent methanol showed the highest antioxidant activity. Camellia sinensis is promising source of natural antioxidants and further studies might be a likely source of its use in remedy of different diseases.
Authors and Affiliations
Humaira Yasmeen , Shahida Hasnain
Keywords
Related Articles
- EP ID EP163853
- DOI -
- Views 99
- Downloads 0
Synthesis, characterization and biological evaluation of tryptamine based benzamide derivatives Kiran
Benzamides and tryptamine are biologically significant compounds, therefore, various benzamide analogous of tryptamine have been efficiently synthesized using tryptamine and different benzoyl chlorides, in order t...
Current status and future prospects of the development of clinical Pharmacy in China: A SWOT analysis
In many industrialized countries, clinical pharmacy has developed into a separate discipline and become a vital part of inpatient care in hospitals. However, as compared to many established branches of medicine, cl...
Larvicidal effect of aqueous and ethanolic extracts of Senna alata on Anopheles gambiae, Culex quinquefasciatus and Aedes aegypti
Senna alata is locally used in South Eastern Nigeria in the treatment of several infections which include ringworm and other parasitic skin diseases.The larvicidal activities of aqueous and ethanolic leaf and stem...
The clinical diagnosis and treatment about 22 cases of limbic encephalitis were retrospectively analyzed
To summarize and analyze the clinical characteristics and treatment of limbic encephalitis, in order to provide the basis for clinical work. We retrospectively analyzed the clinical characteristics, magnetic resona...
Formulation and evaluation of a single vial lyophilized preparation of technetium-99m labeled ethylene dicysteine as a renal scintigraphic agent
99mTc labeled N-N-ethylene-L, L-dicysteine (EC) was introduced in form of multiple-step kit as an alternate renal imaging radiopharmaceutical for commonly used 99mTc-MAG3. We developed a single component lyophilize...