IN VITRO CALLOGENESIS FROM BUD AND STEM EXPLANTS OF DRAGON FRUIT (HYLOCEREUS UNDATUS)
Journal Title: Asian J of Pharm Sci & Tech - Year 2015, Vol 5, Issue 4
Abstract
ABSTRACT This study was carried out to find out the effect of thidiazuron (TDZ) and 6-benzyl aminopurine (BAP) on callus formation of dragon fruit under in vitro conditions for vegetative propagation. Therefore, bud and stem segments of dragon fruit were cultured on Murashige and Skoog (MS) media with two different concentrations of BAP or TDZ for callogenesist under in vitro conditions. The bud explants in MS medium containing 3 mg/l TDZ and 0.5 mg/l NAA exhibited the highest in vitro response compared to other media. Further, result revealed that most responsible plant part for callus formation would be the stem segment. TDZ concentration at 3 mg/l showed the best for callus formation in cultured stem explants of dragon fruit. In this study, TDZ is more effective plant growth regulator than BAP for friable callus production from stem explants of dragon fruit. Key words: BAP, dragon fruit, callus, in vitro culture, TDZ.
Authors and Affiliations
A. Thinesh and T. H. Seran
Keywords
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